Recombinant Mouse Dkk1, His-tagged

• Supplier: Creative Biomart
• Catalog: Dkk1-984M
• Category: Protein
• Species Reactivities: Mouse
• Nature: Recombinant
• Source: NSO Cells
• Tag/Conjugation: His
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Bioactivity: Measured by its ability to interfere with the axis-inducing activity of Xwnt-8 mRNA in early Xenopus embryos. In a population of embryos, the addition of 1 ng of rmDkk-1 per embryo resulted in 60 - 100% reduction in the number of secondary axes produced by 10 - 20 pg of Xwnt-8 mRNA. Optimal concentrations should be determined by each laboratory for each application.Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ND50 for this effect is typically 0.2 - 1 μg/mL in the presence of 10 ng/mL of rmWnt-3a.
• Gene: Dkk1
• Description: Recombinant Mouse Dkk1 (Accession # O54908) Ser30-His272, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.