Active human Acetyl Coenzyme A carboxylase alpha full length protein

Name Active human Acetyl Coenzyme A carboxylase alpha full length protein
Supplier Abcam
Catalog ab196428
Category Protein
Prices $1,311.00
Sizes 10 µg
Applications SDS-PAGE FA
Species Reactivities Human
Nature Recombinant
Source Baculovirus infected sf9 cells
Tag/Conjugation His-DDDDK tag C-Terminus
Purity >= 70 % by SDS-PAGE.
Bioactivity Specific Activity: ≥42.5 pmol/min/µg. A 25 μl Acetyl Coenzyme A carboxylase alpha reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl 2 , 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO 3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected.
SwissProt/Accession Q13085
Gene ACACA
Residue 1 to 2383
Sequence MWWSTLMSILRARSFWKWISTQTVRIIRAVRAHFGGIMDEPSPLAQPLEL NQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLG ISSLQDGLALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFG GNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLK ANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHAS ENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGS GLRVDWQENDFSKRILNVPQELYEKGYVKDVDDGLQAAEEVGYPVMIKAS EGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQIL ADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAK MVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQ IAMGIPLYRIKDIRMMYGVSPWGDSPIDFEDSAHVPCPRGHVIAARITSE NPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSW GENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWL DRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSVSNFLHSLERGQVLPA HTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGG LLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLI QYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGC VLAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNV MNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPN VEKSIKKEMAQYASNITSVLCQFPSQQIANI
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