| Name | BRDT (22-138aa), His, Human |
|---|---|
| Supplier | GenScript |
| Catalog | Z03188-100 |
| Category | Protein |
| Prices | $195.00 |
| Sizes | 100 μg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His |
| Purity | > 95% by SDS-PAGE and HPLC analysis. |
| Bioactivity | Assay condition: 50 mM HEPES, 50 mM NaCl, and 1 mM CHAPS, pH 7.5. Biotinylated acetyl-histone peptide substrate binding to Bromodomain reaction was performed by adding 200 nM substrate to serially diluted BRD, and incubation for 1 h at RT. Treatment with equivalent detection buffer (2 nM Eu-Anti-His and 40 nM U-light-SA in Assay buffer), and incubation for 1 h at RT subsequently followed. |
| Endotoxin | < 1 EU/μg, determined by LAL method. |
| Gene | BRD4 |
| Sequence | MHHHHHHTKK NGRLTNQLQY LQKVVLKDLW KHSFSWPFQR PVDAVKLQLP DYYTIIKNPM DLNTIKKRLE NKYYAKASEC IEDFNTMFSN CYLYNKPGDD IVLMAQALEK LFMQKLSQMP QEEQ |
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