Active human AKT1 full length protein
| Name | Active human AKT1 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab62279 |
| Category | Protein |
| Prices | $239.00 |
| Sizes | 5 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Purity | > 90 % by SDS-PAGE. Purity >90% as determined by SDS-PAGE and Coomassie blue staining. |
| Bioactivity | 124 nmol phosphate incorporated into Akt substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32. |
| SwissProt/Accession | P31749 |
| Gene | AKT1 |
| Sequence | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. |
| Supplier Page | Shop |
Product images
Primary Antibodies: Lane 1: Anti Akt-1 antibody ( ab54752 ) at 5?g/mL dilution. Lane 2: Anti Akt-1 antibody ( ab81283 ) at 1/5000 dilution. Sample: Akt-1 recombinant protein (Tagged)(ab62279) ? 50ng. Secondary Antibody: Lane 1: Goat polyclonal to Mouse IgG ? H&L ? Pre-Adsorbed (HRP) at 1:5000 developed using the ECL technique. Lane 2: Goat polyclonal to Rabbit IgG ? H&L - Pre-Adsorbed (HRP) at 1:5000 developed using the ECL technique. Performed under reducing conditions. Predicted band size : 80kDa. Observed band size : 80kDa. Blocking step: 5% Milk in 50mM TBS+0.05% Tween for 1 hour at RT. Primary antibody buffer: 5% BSA in 50mM TBS+0.05% Tween overnight at 4?C. Secondary antibody buffer: 10% blocking ab126587 in water for 2 hours at RT. Exposure time : 5 minutes
ab62279 Kinase activity plot.
ab62279 on SDS-PAGE. Band at ~85kDa.