All lanes : Anti-alpha smooth muscle Actin antibody ( ab15734 ) at 1 µg/ml Lane 1 : HeLa whole cell lysate Lane 2 : A431 cell lysate Lane 3 : HeLa whole cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/ml Lane 4 : A431 cell lysate with Human alpha smooth muscle Actin peptide (ab16102) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Goat-anti-Rabbit IgG at 1/10000 dilution Performed under reducing conditions. Predicted band size : 42 kDa Observed band size : 42 kDa