Recombinant Mouse Dkk-1 Protein

• Supplier: R&D Systems
• Catalog: 5897-DK
• Category: Protein
• Prices: $329.00
• Sizes: 10 µg
• Species Reactivities: Mouse
• Nature: Recombinant
• Source: Chinese Hamster Ovary cell line, CHO-derived Ser30-His272
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Bioactivity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ND 50 for this effect is 0.02‑0.1 μg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN ). Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 (Catalog # 5897-DK) inhibits a constant dose of 100 ng/mL of Recombinant Human Wnt-3a (Catalog # 5036-WN ). The ED 50 for this effect is 10-60 ng/mL.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• SwissProt/Accession: O54908
• Gene: Dkk1
• Sequence: Ser30 & Thr32

Product References

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