Recombinant Mouse sTNF RI/TNFRSF1A Protein, CF

• Supplier: R&D Systems
• Catalog: 425-R1/CF
• Category: Protein
• Prices: $349.00
• Sizes: 50 µg
• Species Reactivities: Mouse
• Nature: Recombinant
• Source: E. coli -derived Ile22-Ala212, with an N-terminal Met
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Bioactivity: Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED 50 for this effect is 0.1-0.6 µg/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-alpha.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• SwissProt/Accession: P25118
• Gene: Tnfrsf1a
• Sequence: Met

Product References

Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF - Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF

Goodall LJ, Ovecka M, Rycroft D, Friel SL, Sanderson A, Mistry P, Davies ML, Stoop AA. PLoS One. 2015 Sep 9;10(9):e0137065.

Generation and characterization of small single domain antibodies inhibiting - Generation and characterization of small single domain antibodies inhibiting

Steeland S, Puimege L, Vandenbroucke RE, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C. J Biol Chem. 2015 Feb 13;290(7):4022-37.

Glial tumor necrosis factor alpha (TNFalpha) generates metaplastic inhibition of - Glial tumor necrosis factor alpha (TNFalpha) generates metaplastic inhibition of

Huie JR, Baumbauer KM, Lee KH, Bresnahan JC, Beattie MS, Ferguson AR, Grau JW. PLoS One. 2012;7(6):e39751.

Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by - Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by

Mitchell HM, White DM, Domowicz MS, Kraig RP. J Neurochem. 2011 Apr;117(2):187-96.

Tumor necrosis factor-alpha mediates one component of competitive, - Tumor necrosis factor-alpha mediates one component of competitive,

Kaneko M, Stellwagen D, Malenka RC, Stryker MP. Neuron. 2008 Jun 12;58(5):673-80.

P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor - P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor

Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P. J Biol Chem. 2006 Oct 13;281(41):30684-96. Epub 2006 Aug 1.

Circulating cytokine/inhibitor profiles reshape the understanding of the - Circulating cytokine/inhibitor profiles reshape the understanding of the

Osuchowski MF, Welch K, Siddiqui J, Remick DG. J Immunol. 2006 Aug 1;177(3):1967-74.

Sequential ELISA to profile multiple cytokines from small volumes. - Sequential ELISA to profile multiple cytokines from small volumes.

Osuchowski MF, Siddiqui J, Copeland S, Remick DG. J Immunol Methods. 2005 Jul;302(1-2):172-81.

Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor - Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor

LaBue M, Colburn KK, Green LM. Thyroid. 2004 Apr;14(4):249-62.

A role for interferon-gamma in focal cerebral ischemia in mice. - A role for interferon-gamma in focal cerebral ischemia in mice.

Lambertsen KL, Gregersen R, Meldgaard M, Clausen BH, Heibol EK, Ladeby R, Knudsen J, Frandsen A, Owens T, Finsen B. J Neuropathol Exp Neurol. 2004 Sep;63(9):942-55.