Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF
| Name | Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF |
|---|---|
| Supplier | R&D Systems |
| Catalog | 1765-DK/CF |
| Category | Protein |
| Prices | $329.00 |
| Sizes | 10 µg |
| Species Reactivities | Mouse |
| Nature | Recombinant |
| Source | Mouse myeloma cell line, NS0-derived Ser30-His272, with a C-terminal 10-His tag |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Bioactivity | Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ND 50 for this effect is 0.2‑1 μg/mL in the presence of 10 ng/mL of rmWnt-3a. Measured by its ability to interfere with the axis-inducing activity of Xwnt-8 mRNA in early Xenopus embryos. In a population of embryos, the addition of 1 ng of rmDkk-1 per embryo resulted in 60 ‑ 100% reduction in the number of secondary axes produced by 10-20 pg of Xwnt-8 mRNA. Optimal concentrations should be determined by each laboratory for each application. |
| Endotoxin | <1.0 EU per 1 μg of the protein by the LAL method. |
| SwissProt/Accession | O54908 |
| Gene | Dkk1 |
| Sequence | Ser30 |
| Supplier Page | Shop |
Product images
Recombinant Mouse Dkk-1 (Catalog # 5439-DK) inhibits Wnt-3a-induced alkaline phosphatase production by the MC3T3‑E1 mouse preosteoblast cell line. The ED 50 for this effect is 0.02-0.1 µg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).
1 µg/lane of Recombinant Mouse Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 35-40 kDa. Multiple bands in gel are due to variable glycosylation.
Product References
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