Recombinant Mouse Dkk-1 (Histidine-tagged) Protein

Name Recombinant Mouse Dkk-1 (Histidine-tagged) Protein
Supplier R&D Systems
Catalog 1765-DK
Category Protein
Prices $329.00
Sizes 10 µg
Species Reactivities Mouse
Nature Recombinant
Source Mouse myeloma cell line, NS0-derived Ser30-His272, with a C-terminal 10-His tag
Purity >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Bioactivity Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 (Catalog # 1765-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). The ED 50 for this effect is 30-180 ng/mL. Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ND 50 for this effect is 0.2-1 μg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN ).
Endotoxin <1.0 EU per 1 μg of the protein by the LAL method.
SwissProt/Accession O54908
Gene Dkk1
Sequence Ser30
Supplier Page Shop

Product References

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