Recombinant MLL1 Complex
| Name | Recombinant MLL1 Complex |
|---|---|
| Supplier | ActiveMotif |
| Catalog | 31423 |
| Prices | $415.00, $3,500.00 |
| Sizes | 20 µg, 1 mg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Gene | KMT2A |
| Description | Reconstituted MLL1 complex contains amino acids 3735 - 3973 of the human MLL1 protein (accession number NP_001184033.1) with N-terminal GST tag and MW= 53.7 kDa; full length human WDR5 (GenBank Accession No.NM_017588), with N-terminal 6×His tag and MW= 35 kDa; full length human ASH2L (GenBank Accession No. NM_001105214), N-terminal 6×His tag and MW= 61 kDa; full length human RbBP5 (GenBank Accession No. NM_005057), N-terminal 6×His-tag and MW= 60kDa, and full length human DPY30 (GenBank Accession No. NM_032574), N-terminal 6×His-tag and MW=12 kDa, all individually expressed in E.coli cells |
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Product images
Recombinant MLL1 complex gel. Recombinant MLL1 complex was run on an 12% SDS-PAGE gel and stained with Coomassie blue.
Recombinant MLL1 complex activity assay. HMT assay measuring the activity of Recombinant MLL1 Complex. Activity was detected by MALDI-TOF. Catalytic capacity: >300 turnover/ enzyme molecule
Recombinant MLL1 complex activity assay. 3. 3 μM H3K4me0 (1-21aa) peptide was incubated with MLL1 Complex in reaction buffer for 3 hour at room temperature. MLL1 Complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.