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Cdc20 Antibody (E-7): sc-13162
- Cdc20 Antibody (E-7) is a mouse monoclonal IgG1 κ Cdc20 antibody, cited in 105 publications, provided at 200 µg/ml
- raised against amino acids 1-175 of Cdc20 of human origin
- Cdc20 Antibody (E-7) is recommended for detection of Cdc20 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-Cdc20 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to TRITC for IF, IHC(P) and FCM
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc20 (E-7): sc-13162.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc20 Antibody (E-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc20 Antibody (E-7) (see ordering information below).
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Cdc20 Antibody (E-7) is an IgG1 κ mouse monoclonal Cdc20 antibody (also designated p55 CDC antibody or Cell division cycle protein 20 antibody) that detects the p55 CDC protein of mouse, rat and human origin by WB, IP, IF and IHC(P). Cdc20 Antibody (E-7) is available as both the non-conjugated anti-Cdc20 antibody form, as well as multiple conjugated forms of anti-Cdc20 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13suc 1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase termed Cdc20 exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The Cdc20 transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested. Cdc20 shows kinase activity towards a-casein and myelin basic protein.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Human p55(CDC)/Cdc20 associates with cyclin A and is phosphorylated by the cyclin A-Cdk2 complex. | Ohtoshi, A., et al. 2000. Biochem Biophys Res Commun. 268: 530-4. PMID: 10679238
- Oscillation of MPF is accompanied by periodic association between cdc25 and cdc2-cyclin B. | Jessus, C. and Beach, D. 1992. Cell. 68: 323-32. PMID: 1310257
- Regulation of neuronal cdc20 (p55cdc) expression by the plasticity-related transcription factor zif268. | Conway, AM., et al. 2007. Synapse. 61: 463-8. PMID: 17372985
- Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. | Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
- Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2. | Pines, J. and Hunter, T. 1989. Cell. 58: 833-46. PMID: 2570636
- cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF. | Arion, D., et al. 1988. Cell. 55: 371-8. PMID: 2844417
- The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis. | Dunphy, WG., et al. 1988. Cell. 54: 423-31. PMID: 3293802
- p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase. | Brizuela, L., et al. 1987. EMBO J. 6: 3507-14. PMID: 3322810
- A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4. | Weinstein, J., et al. 1994. Mol Cell Biol. 14: 3350-63. PMID: 7513050
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc20 Antibody (E-7) | sc-13162 | 200 µg/ml | $310.00 | |||
Cdc20 Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-528280 | 200 µg Ab; 10 µg BP | $348.00 | |||
Cdc20 Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-520619 | 200 µg Ab, 40 µg BP | $348.00 | |||
Cdc20 Antibody (E-7): m-IgG1 BP-HRP Bundle | sc-542850 | 200 µg Ab; 20 µg BP | $348.00 | |||
Cdc20 Antibody (E-7) AC | sc-13162 AC | 500 µg/ml, 25% agarose | $408.00 | |||
Cdc20 Antibody (E-7) HRP | sc-13162 HRP | 200 µg/ml | $310.00 | |||
Cdc20 Antibody (E-7) FITC | sc-13162 FITC | 200 µg/ml | $324.00 | |||
Cdc20 Antibody (E-7) PE | sc-13162 PE | 200 µg/ml | $337.00 | |||
Cdc20 Antibody (E-7) Alexa Fluor® 488 | sc-13162 AF488 | 200 µg/ml | $350.00 | |||
Cdc20 Antibody (E-7) Alexa Fluor® 546 | sc-13162 AF546 | 200 µg/ml | $350.00 | |||
Cdc20 Antibody (E-7) Alexa Fluor® 594 | sc-13162 AF594 | 200 µg/ml | $350.00 | |||
Cdc20 Antibody (E-7) Alexa Fluor® 647 | sc-13162 AF647 | 200 µg/ml | $350.00 | |||
Cdc20 Antibody (E-7) Alexa Fluor® 680 | sc-13162 AF680 | 200 µg/ml | $350.00 | |||
Cdc20 Antibody (E-7) Alexa Fluor® 790 | sc-13162 AF790 | 200 µg/ml | $350.00 | |||
Cdc20 Antibody (E-7) TRITC | sc-13162 TRITC | 200 µg/ml | $314.00 |