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CREB1 Antibody (D-12): sc-377154
- CREB1 Antibody (D-12) is a mouse monoclonal IgG2a κ CREB1 antibody, cited in 57 publications, provided at 200 µg/ml
- raised against amino acids 254-327 of CREB-1 of human origin
- CREB1 Antibody (D-12) is recommended for detection of CREB-1A, CREB-1B, CREM and ATF-1 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-CREB1 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-377154 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CREB1 (D-12): sc-377154.
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CREB1 Antibody (D-12) for WB and IHC(P) applications. These reagents are now offered in bundles with CREB1 Antibody (D-12) (see ordering information below).
CREB-1 Antibody (D-12) is an IgG2a κ mouse monoclonal CREB-1 antibody (also designated CREB antibody or CREB1 antibody) that detects the CREB-1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. CREB-1 Antibody (D-12) is available as both the non-conjugated anti-CREB-1 antibody form, as well as multiple conjugated forms of anti-CREB-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis-acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contains highly divergent N-terminal domains, but shares a C-terminal leucine zipper for dimerization and DNA binding. Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription. Phosphorylation of CREB on Ser 133 by protein kinase A facilitates its interaction with the CREB-binding protein (CBP) and activates the basal transcription complex. CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1. CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- CREB regulates hepatic gluconeogenesis through the coactivator PGC-1. | Herzig, S., et al. 2001. Nature. 413: 179-83. PMID: 11557984
- Assignment of the human gene for CREB1 to chromosome 2q32.3-q34. | Taylor, AK., et al. 1990. Genomics. 7: 416-21. PMID: 2142119
- Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers. | Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
- Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters. | Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
- Identification of a cyclic-AMP-responsive element within the rat somatostatin gene. | Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
- Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB. | Yamamoto, KK., et al. 1988. Nature. 334: 494-8. PMID: 2900470
- Nuclear protein CBP is a coactivator for the transcription factor CREB. | Kwok, RP., et al. 1994. Nature. 370: 223-6. PMID: 7913207
- Activation of cAMP and mitogen responsive genes relies on a common nuclear factor. | Arias, J., et al. 1994. Nature. 370: 226-9. PMID: 8028671
- Dilated cardiomyopathy in transgenic mice expressing a dominant-negative CREB transcription factor in the heart. | Fentzke, RC., et al. 1998. J Clin Invest. 101: 2415-26. PMID: 9616213
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CREB1 Antibody (D-12) | sc-377154 | 200 µg/ml | $310.00 | |||
CREB1 Antibody (D-12): m-IgGκ BP-HRP Bundle | sc-523213 | 200 µg Ab, 40 µg BP | $348.00 | |||
CREB1 Antibody (D-12): m-IgG2a BP-HRP Bundle | sc-546150 | 200 µg Ab; 10 µg BP | $348.00 | |||
CREB1 Antibody (D-12) X | sc-377154 X | 200 µg/0.1 ml | $310.00 | |||
CREB1 Antibody (D-12) AC | sc-377154 AC | 500 µg/ml, 25% agarose | $408.00 | |||
CREB1 Antibody (D-12) HRP | sc-377154 HRP | 200 µg/ml | $310.00 | |||
CREB1 Antibody (D-12) FITC | sc-377154 FITC | 200 µg/ml | $324.00 | |||
CREB1 Antibody (D-12) PE | sc-377154 PE | 200 µg/ml | $337.00 | |||
CREB1 Antibody (D-12) Alexa Fluor® 488 | sc-377154 AF488 | 200 µg/ml | $350.00 | |||
CREB1 Antibody (D-12) Alexa Fluor® 546 | sc-377154 AF546 | 200 µg/ml | $350.00 | |||
CREB1 Antibody (D-12) Alexa Fluor® 594 | sc-377154 AF594 | 200 µg/ml | $350.00 | |||
CREB1 Antibody (D-12) Alexa Fluor® 647 | sc-377154 AF647 | 200 µg/ml | $350.00 | |||
CREB1 Antibody (D-12) Alexa Fluor® 680 | sc-377154 AF680 | 200 µg/ml | $350.00 | |||
CREB1 Antibody (D-12) Alexa Fluor® 790 | sc-377154 AF790 | 200 µg/ml | $350.00 |