GLI-2 Antibody (C-10): sc-271786

GLI-2 Antibody (C-10) is an IgG₁ κ mouse monoclonal GLI-2 antibody (also designated GLI2 antibody) that detects the GLI-2 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. GLI-2 Antibody (C-10) is available as both the non-conjugated anti-GLI-2 antibody form, as well as multiple conjugated forms of anti-GLI-2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GLI-2 (GLI family zinc finger 2), also known as HPE9 or THP (tax helper protein), is a 1,586 amino acid nuclear protein that acts as a transcriptional activator and belongs to the GLI C2H2-type zinc-finger protein family. Localized to the nucleus, GLI-2 is thought to play a role in embryogenesis. The gene encoding GLI-2 maps to human chromosome 2q14.2, and when defective is the cause of holoprosencephaly type 9 (HPE9). GLI-2 exists as five alternatively spliced isoforms.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Loss-of-function mutations in the human GLI2 gene are associated with pituitary anomalies and holoprosencephaly-like features.  |  Roessler, E., et al. 2003. Proc Natl Acad Sci U S A. 100: 13424-9. PMID: 14581620
2. A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2.  |  Roessler, E., et al. 2005. Hum Mol Genet. 14: 2181-8. PMID: 15994174
3. A family of human zinc finger proteins that bind methylated DNA and repress transcription.  |  Filion, GJ., et al. 2006. Mol Cell Biol. 26: 169-81. PMID: 16354688
4. [Progress in the study of KRAB zinc finger protein].  |  Tian, CY., et al. 2006. Yi Chuan. 28: 1451-6. PMID: 17098717
5. Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway.  |  Maloverjan, A., et al. 2010. Exp Cell Res. 316: 627-37. PMID: 19878745
6. The GLI-Kruppel family of human genes.  |  Ruppert, JM., et al. 1988. Mol Cell Biol. 8: 3104-13. PMID: 2850480
7. A new regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1 and cloning of cDNAs encoding its binding proteins.  |  Tanimura, A., et al. 1993. J Virol. 67: 5375-82. PMID: 8350401
8. Cloning of novel isoforms of the human Gli2 oncogene and their activities to enhance tax-dependent transcription of the human T-cell leukemia virus type 1 genome.  |  Tanimura, A., et al. 1998. J Virol. 72: 3958-64. PMID: 9557682

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GLI-2 Antibody (C-10) has 48 citations in a variety of scientific publications.

Primary antibodies like Anti-GLI-2 Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GLI-2 Antibody (C-10) Specifications

Alternate Product Name

GLI2

Product Clone Name

C-10

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

841-1140

Epitope Target

GLI-2

Epitope Species

human

Product Reactivity/Detection

GLI-2

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

48 citations in various scientific publications

Recommended Secondary Detection Reagents


Mouse IgG₁ Binding Proteins

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent