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YWHAB Polyclonal antibody

YWHAB Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10936-1-AP

Synonyms

14 3 3, 14 3 3 alpha, 14 3 3 protein beta, 14 3 3 protein beta alpha, 14 3 3 protein beta/alpha, YWHAB

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YWHAB Polyclonal antibody (10936-1-AP)

    IHC staining of human colon cancer using 10936-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YWHAB Polyclonal antibody (10936-1-AP)

    IHC staining of human colon cancer using 10936-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YWHAB Polyclonal antibody (10936-1-AP)

    IHC staining of human colon cancer using 10936-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YWHAB Polyclonal antibody (10936-1-AP)

    IHC staining of human colon cancer using 10936-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YWHAB Polyclonal antibody (10936-1-AP)

    IF Staining of HepG2 using 10936-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using YWHAB antibody (10936-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10936-1-AP targets YWHAB in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YWHAB fusion protein Ag1383
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC010352
Gene Symbol YWHAB
Gene ID (NCBI) 7529
RRIDAB_2217489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YWHAB (also known as 14-3-3 beta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised agaist the N-terminal region of human YWHAB.

Protocols

Product Specific Protocols
IHC protocol for YWHAB antibody 10936-1-APDownload protocol
IF protocol for YWHAB antibody 10936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols