HNF-3α Antibody (Q-6): sc-101058

HNF-3α Antibody (Q-6) is an IgG2a κ mouse monoclonal HNF-3α antibody (also designated HNF-3α antibody or FOXA1 antibody) that detects HNF-3α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. HNF-3α Antibody (Q-6) is available as the non-conjugated anti-HNF-3α antibody form. HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation. HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4α is expressed in liver, kidney, pancreas, small intestine, testis and colon; HNF-4γ is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin.

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References:

1. Hepatic nuclear factor-3 (HNF-3 or Foxa2) regulates glucagon gene transcription by binding to the G1 and G2 promoter elements.  |  Gauthier, BR., et al. 2002. Mol Endocrinol. 16: 170-83. PMID: 11773447
2. Adenovirus-mediated increase in HNF-3beta or HNF-3alpha shows differences in levels of liver glycogen and gene expression.  |  Tan, Y., et al. 2002. Hepatology. 35: 30-9. PMID: 11786957
3. HNF-3beta is a critical factor for the expression of the Jaagsiekte sheep retrovirus long terminal repeat in type II pneumocytes but not in Clara cells.  |  McGee-Estrada, K., et al. 2002. Virology. 292: 87-97. PMID: 11878911
4. Elevated hepatocyte levels of the Forkhead box A2 (HNF-3beta) transcription factor cause postnatal steatosis and mitochondrial damage.  |  Hughes, DE., et al. 2003. Hepatology. 37: 1414-24. PMID: 12774021
5. Transcription factor HNF-6/OC-1 inhibits the stimulation of the HNF-3alpha/Foxa1 gene by TGF-beta in mouse liver.  |  Plumb-Rudewiez, N., et al. 2004. Hepatology. 40: 1266-74. PMID: 15562441
6. More potent transcriptional activators or a transdominant inhibitor of the HNF1 homeoprotein family are generated by alternative RNA processing.  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
7. The HNF-3 gene family of transcription factors in mice: gene structure, cDNA sequence, and mRNA distribution.  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
8. Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes.  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
9. The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression.  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HNF-3α Antibody (Q-6) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-HNF-3α Antibody (Q-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HNF-3α Antibody (Q-6) Specifications

Alternate Product Name

FOXA1

Product Clone Name

Q-6

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

367-473

Epitope Target

HNF-3α

Epitope Species

human

Product Reactivity/Detection

HNF-3α

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

32 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

N/A

The preferred HNF-3α/β antibody is HNF-3α/β Antibody (E-4): sc-377033.