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IκB-β Antibody (D-3): sc-74451
- IκB-β Antibody (D-3) is a mouse monoclonal IgG2a κ IκB-β antibody, cited in 7 publications, provided at 200 µg/ml
- raised against amino acids 1-359 representing full length IκB-β of mouse origin
- I kappa B-beta Antibody (D-3) is recommended for detection of IκB-β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-I kappa B-beta Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-74451 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IκB-β (D-3): sc-74451.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IκB-β Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with IκB-β Antibody (D-3) (see ordering information below).
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IκB-β Antibody (D-3) is an IgG2a κ mouse monoclonal I kappa B-beta antibody (also designated IKBB antibody, NFKB Inhibitor Beta antibody, NF-Kappa-B Inhibitor Beta antibody, TRIP9 antibody, IKBB antibody, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor Beta antibody, Thyroid Receptor-Interacting Protein 9 antibody, TR-Interacting Protein 9 antibody, I-Kappa-B-Beta antibody, NF-Kappa-BIB antibody, IkappaBbeta antibody, IkB-Beta antibody, TRIP-9 antibody, or IkB-B antibody) that detects the I kappa B-beta protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. IκB-β Antibody (D-3) is available as both the non-conjugated anti-I kappa B-beta antibody form, as well as multiple conjugated forms of anti-I kappa B-beta antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- I kappa B gamma, a 70 kd protein identical to the C-terminal half of p110 NF-kappa B: a new member of the I kappa B family. | Inoue, J., et al. 1992. Cell. 68: 1109-20. PMID: 1339305
- Reduced skeletal muscle inhibitor of kappaB beta content is associated with insulin resistance in subjects with type 2 diabetes: reversal by exercise training. | Sriwijitkamol, A., et al. 2006. Diabetes. 55: 760-7. PMID: 16505240
- Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity. | Haskill, S., et al. 1991. Cell. 65: 1281-9. PMID: 1829648
- Rel-associated pp40: an inhibitor of the rel family of transcription factors. | Davis, N., et al. 1991. Science. 253: 1268-71. PMID: 1891714
- The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation. | Kerr, LD., et al. 1991. Genes Dev. 5: 1464-76. PMID: 1907941
- Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B. | Ghosh, S. and Baltimore, D. 1990. Nature. 344: 678-82. PMID: 2157987
- I kappa B-beta regulates the persistent response in a biphasic activation of NF-kappa B. | Thompson, JE., et al. 1995. Cell. 80: 573-82. PMID: 7867065
- I kappa B epsilon, a novel member of the I kappa B family, controls RelA and cRel NF-kappa B activity. | Whiteside, ST., et al. 1997. EMBO J. 16: 1413-26. PMID: 9135156
- Cloning and functional characterization of mouse IkappaBepsilon. | Simeonidis, S., et al. 1997. Proc Natl Acad Sci U S A. 94: 14372-7. PMID: 9405619
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IκB-β Antibody (D-3) | sc-74451 | 200 µg/ml | $310.00 | |||
IκB-β Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-528667 | 200 µg Ab; 10 µg BP | $348.00 | |||
IκB-β Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-521114 | 200 µg Ab, 40 µg BP | $348.00 | |||
IκB-β Antibody (D-3): m-IgG2a BP-HRP Bundle | sc-547138 | 200 µg Ab; 10 µg BP | $348.00 | |||
IκB-β Antibody (D-3) X | sc-74451 X | 200 µg/0.1 ml | $310.00 | |||
IκB-β Antibody (D-3) AC | sc-74451 AC | 500 µg/ml, 25% agarose | $408.00 | |||
IκB-β Antibody (D-3) HRP | sc-74451 HRP | 200 µg/ml | $310.00 | |||
IκB-β Antibody (D-3) FITC | sc-74451 FITC | 200 µg/ml | $324.00 | |||
IκB-β Antibody (D-3) PE | sc-74451 PE | 200 µg/ml | $337.00 | |||
IκB-β Antibody (D-3) Alexa Fluor® 488 | sc-74451 AF488 | 200 µg/ml | $350.00 | |||
IκB-β Antibody (D-3) Alexa Fluor® 546 | sc-74451 AF546 | 200 µg/ml | $350.00 | |||
IκB-β Antibody (D-3) Alexa Fluor® 594 | sc-74451 AF594 | 200 µg/ml | $350.00 | |||
IκB-β Antibody (D-3) Alexa Fluor® 647 | sc-74451 AF647 | 200 µg/ml | $350.00 | |||
IκB-β Antibody (D-3) Alexa Fluor® 680 | sc-74451 AF680 | 200 µg/ml | $350.00 | |||
IκB-β Antibody (D-3) Alexa Fluor® 790 | sc-74451 AF790 | 200 µg/ml | $350.00 |