NFATc2 Antibody (4G6-G5): sc-7296

NFATc2 Antibody (4G6-G5) is an IgG_2a κ mouse monoclonal NFATc2 antibody (also designated NFAT1 antibody, or nuclear factor of activated T cells 2 antibody) that detects the NFATc2 protein of mouse, rat and human origin by WB, IP and IHC(P). NFATc2 Antibody (4G6-G5) is available as both the non-conjugated anti-NFATc2 antibody form, as well as multiple conjugated forms of anti-NFATc2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The NFAT (nuclear factor of activated T cells) family of transcription factors regulates cytokine expression in T cells. Members of the family include NFATc1 (NFATc), NFATc2 (NFATp), NFATn, NFATc3 (NFAT4, NFATx) and NFATc4 (NFAT3). Recognition of antigen by the T cell receptor (TCR) eventually activates the calcium-dependent protein phosphatase calcineurin. Once activated, calcineurin stimulates the translocation of NFATc1 (cytoplasmic) from the cytoplasm to the nucleus where it associates with NFATn (nuclear). Like NFATc1, NFATc2 resides in the cytoplasm and translocates to the nucleus subsequent to activation of calcineurin. Once in the nucleus, NFATc2 synergizes with AP-1 transcription factors to initiate transcription of cytokine genes. NFATc3 and NFATc4 share 65% sequence identity with other members of the NFAT family. They are similar to NFATc2 in that they also synergize with the AP-1 family of proteins.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes.  |  Liu, J., et al. 1991. Cell. 66: 807-15. PMID: 1715244
2. Nuclear association of a T-cell transcription factor blocked by FK-506 and cyclosporin A.  |  Flanagan, WM., et al. 1991. Nature. 352: 803-7. PMID: 1715516
3. Cot/Tpl2 and PKCzeta cooperate in the regulation of the transcriptional activity of NFATc2 through the phosphorylation of its amino-terminal domain.  |  Gómez-Casero, E., et al. 2007. Cell Signal. 19: 1652-61. PMID: 17398070
4. Cyclosporin A specifically inhibits function of nuclear proteins involved in T cell activation.  |  Emmel, EA., et al. 1989. Science. 246: 1617-20. PMID: 2595372
5. NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity.  |  Ho, SN., et al. 1995. J Biol Chem. 270: 19898-907. PMID: 7650004
6. NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus.  |  Masuda, ES., et al. 1995. Mol Cell Biol. 15: 2697-706. PMID: 7739550
7. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins.  |  Hoey, T., et al. 1995. Immunity. 2: 461-72. PMID: 7749981
8. NF-AT components define a family of transcription factors targeted in T-cell activation.  |  Northrop, JP., et al. 1994. Nature. 369: 497-502. PMID: 8202141
9. The T-cell transcription factor NFATp is a substrate for calcineurin and interacts with Fos and Jun.  |  Jain, J., et al. 1993. Nature. 365: 352-5. PMID: 8397339

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NFATc2 Antibody (4G6-G5) has 151 citations in a variety of scientific publications.

Primary antibodies like Anti-NFATc2 Antibody (4G6-G5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NFATc2 Antibody (4G6-G5) Specifications

Alternate Product Name

NFATc2

Product Clone Name

4G6-G5

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

433-567

Epitope Target

NFATc2

Epitope Species

mouse

Product Reactivity/Detection

NFATc2

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IHC(P)

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

151 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG_2a Binding Proteins

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent