NTH1 Antibody (2660C1a): sc-130644

NTH1 Antibody (2660C1a) is an IgG₁ mouse monoclonal NTH1 antibody (also designated NTH1 antibody) that detects the NTH1 protein of mouse, rat and human origin by WB and IP. NTH1 Antibody (2660C1a) is available as the non-conjugated anti-NTH1 antibody. The human endonuclease III (hNTH1), a homolog of the E. Coli enzyme (Nth), is a DNA glycosylase with abasic (apurinic/ apyrimidinic (AP)) lyase activity that specifically cleaves oxidatively damaged pyrimidines in DNA. The enzyme carries out beta-elimination and forms a Schiff base between the active site at Lysine-212 and the deoxyribose generated after base removal. Full-length human NTH1 sorts exclusively into the nuclei, whereas most mouse NTH1 protein sorts into the mitochondria, with a relatively small amount localized in the nuclei. This difference is due to the presence of a nuclear localization sequence in the human NTH1 that is absent in the mouse form of the protein. The mammalian NTH1 gene lies immediately adjacent to one of the tuberous sclerosis disease-determining genes, TSC2, in a head-to-head orientation. The two genes share a promoter with bidirectional activity essential for the transcription of both genes. DNA glycosylases such as NTH1 play an important role in the excision of damaged bases in the genome.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Identification of functional elements in the bidirectional promoter of the mouse Nthl1 and Tsc2 genes.  |  Ikeda, S., et al. 2000. Biochem Biophys Res Commun. 273: 1063-8. PMID: 10891372
2. Differential intracellular localization of the human and mouse endonuclease III homologs and analysis of the sorting signals.  |  Ikeda, S., et al. 2002. DNA Repair (Amst). 1: 847-54. PMID: 12531031
3. Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue.  |  Ikeda, S., et al. 1998. J Biol Chem. 273: 21585-93. PMID: 9705289
4. Cloning and characterization of a mouse homologue (mNthl1) of Escherichia coli endonuclease III.  |  Sarker, AH., et al. 1998. J Mol Biol. 282: 761-74. PMID: 9743625
5. The discovery of a new family of mammalian enzymes for repair of oxidatively damaged DNA, and its physiological implications.  |  Hazra, TK., et al. 2003. Carcinogenesis. 24: 155-7. PMID: 12584162

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NTH1 Antibody (2660C1a) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NTH1 Antibody (2660C1a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NTH1 Antibody (2660C1a) Specifications

Alternate Product Name

NTH1

Product Clone Name

2660C1a

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁

Amino Acids

Epitope Target

NTH1

Epitope Species

human

Product Reactivity/Detection

NTH1

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP

Vialing Information

Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations

1 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

N/A