Sox-6 Antibody (A-4): sc-393314

Sox-6 Antibody (A-4) is an IgG_2b κ mouse monoclonal Sox-6 antibody (also designated SOX6 antibody or SRY-related HMG-box transcription factor 6 antibody) that detects the Sox-6 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Sox-6 Antibody (A-4) is available as both the non-conjugated anti-Sox-6 antibody form, as well as multiple conjugated forms of anti-Sox-6 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Identification of a novel Sry-related gene and its germ cell-specific expression.  |  Osaki, E., et al. 1999. Nucleic Acids Res. 27: 2503-10. PMID: 10359848
2. Roles of Sox factors in neural determination: conserved signaling in evolution?  |  Sasai, Y. 2001. Int J Dev Biol. 45: 321-6. PMID: 11291862
3. Ancestry and diversity of the HMG box superfamily.  |  Laudet, V., et al. 1993. Nucleic Acids Res. 21: 2493-501. PMID: 8506143
4. Sox10, a novel transcriptional modulator in glial cells.  |  Kuhlbrodt, K., et al. 1998. J Neurosci. 18: 237-50. PMID: 9412504
5. Characterisation and mapping of the human SOX14 gene.  |  Arsic, N., et al. 1998. Cytogenet Cell Genet. 83: 139-46. PMID: 9925951

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sox-6 Antibody (A-4) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Sox-6 Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sox-6 Antibody (A-4) Specifications

Alternate Product Name

SOX6

Product Clone Name

A-4

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2b kappa light chain

Amino Acids

506-600

Epitope Target

Sox-6

Epitope Species

human

Product Reactivity/Detection

Sox-6

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

8 citations in various scientific publications

Recommended Secondary Detection Reagents


Mouse IgG_2b Binding Proteins

• m-IgG_2b BP-HRP: sc-542741 (Mouse IgG_2b binding protein conjugated to horseradish peroxidase)
• m-IgG_2b BP-B: sc-542744 (Mouse IgG_2b binding protein conjugated to biotin)
• m-IgG_2b BP-FITC: sc-542743 (Mouse IgG_2b binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2b BP-CFL 488: sc-542745 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 488)
• m-IgG_2b BP-CFL 555: sc-542746 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 555)
• m-IgG_2b BP-CFL 594: sc-542747 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 594)
• m-IgG_2b BP-CFL 647: sc-542748 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 647)
• m-IgG_2b BP-CFL 680: sc-542749 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 680)
• m-IgG_2b BP-CFL 790: sc-542750 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 790)
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Mouse IgG Kappa Light Chain Binding Proteins
• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)



Available Conjugates/Forms




AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent




Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-Sox-6 Antibody (A-4) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.