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TCF-4 Antibody (D-4): sc-166699
- TCF-4 Antibody (D-4) is a mouse monoclonal IgG1 κ TCF-4 antibody, cited in 47 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 565-592 at the C-terminus of TCF-4 of human origin
- TCF-4 Antibody (D-4) is recommended for detection of TCF-4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TCF-4 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-166699 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TCF-4 (D-4): sc-166699.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TCF-4 Antibody (D-4) for WB applications. These reagents are now offered in bundles with TCF-4 Antibody (D-4) (see ordering information below).
TCF-4 Antibody (D-4) is an IgG1 κ mouse monoclonal TCF-4 antibody (also designated TCF4 antibody, TCF7L2 antibody, or transcription factor 7 like 2 antibody) that detects the TCF-4 protein of mouse, rat and human origin by WB, IP, IF and ELISA. TCF-4 Antibody (D-4) is available as both the non-conjugated anti-TCF-4 antibody form, as well as multiple conjugated forms of anti-TCF-4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to β-catenin in a cooperative manner. This TCF-4/β-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes. c-Jun also interacts with TCF-4 and β-catenin, and the phosphorylation-dependent interaction between c-Jun and TCF4 regulates intestinal tumorigenesis by integrating JNK and APC/β-catenin. TCF-4 is also implicated in bipolar affective disorder.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells. | Staal, FJ., et al. 1999. Int Immunol. 11: 317-23. PMID: 10221643
- The human T cell transcription factor-1 gene. Structure, localization, and promoter characterization. | van de Wetering, M., et al. 1992. J Biol Chem. 267: 8530-6. PMID: 1569101
- Human immunodeficiency virus-restricted replication in astrocytes and the ability of gamma interferon to modulate this restriction are regulated by a downstream effector of the Wnt signaling pathway. | Carroll-Anzinger, D., et al. 2007. J Virol. 81: 5864-71. PMID: 17392368
- Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box. | van de Wetering, M., et al. 1991. EMBO J. 10: 123-32. PMID: 1989880
- An HMG-box-containing T-cell factor required for thymocyte differentiation. | Verbeek, S., et al. 1995. Nature. 374: 70-4. PMID: 7870176
- Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. | Morin, PJ., et al. 1997. Science. 275: 1787-90. PMID: 9065402
- Wnt-1 induces growth, cytosolic beta-catenin, and Tcf/Lef transcriptional activation in Rat-1 fibroblasts. | Young, CS., et al. 1998. Mol Cell Biol. 18: 2474-85. PMID: 9566868
- Control of neural crest cell fate by the Wnt signalling pathway. | Dorsky, RI., et al. 1998. Nature. 396: 370-3. PMID: 9845073
- Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium. | Barker, N., et al. 1999. Am J Pathol. 154: 29-35. PMID: 9916915
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCF-4 Antibody (D-4) | sc-166699 | 200 µg/ml | $310.00 | |||
TCF-4 Antibody (D-4): m-IgG Fc BP-HRP Bundle | sc-529033 | 200 µg Ab; 10 µg BP | $348.00 | |||
TCF-4 Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-521715 | 200 µg Ab, 40 µg BP | $348.00 | |||
TCF-4 Antibody (D-4): m-IgG1 BP-HRP Bundle | sc-543280 | 200 µg Ab; 20 µg BP | $348.00 | |||
TCF-4 Antibody (D-4) X | sc-166699 X | 200 µg/0.1 ml | $310.00 | |||
TCF-4 Antibody (D-4) AC | sc-166699 AC | 500 µg/ml, 25% agarose | $408.00 | |||
TCF-4 Antibody (D-4) HRP | sc-166699 HRP | 200 µg/ml | $310.00 | |||
TCF-4 Antibody (D-4) FITC | sc-166699 FITC | 200 µg/ml | $324.00 | |||
TCF-4 Antibody (D-4) PE | sc-166699 PE | 200 µg/ml | $337.00 | |||
TCF-4 Antibody (D-4) Alexa Fluor® 488 | sc-166699 AF488 | 200 µg/ml | $350.00 | |||
TCF-4 Antibody (D-4) Alexa Fluor® 546 | sc-166699 AF546 | 200 µg/ml | $350.00 | |||
TCF-4 Antibody (D-4) Alexa Fluor® 594 | sc-166699 AF594 | 200 µg/ml | $350.00 | |||
TCF-4 Antibody (D-4) Alexa Fluor® 647 | sc-166699 AF647 | 200 µg/ml | $350.00 | |||
TCF-4 Antibody (D-4) Alexa Fluor® 680 | sc-166699 AF680 | 200 µg/ml | $350.00 | |||
TCF-4 Antibody (D-4) Alexa Fluor® 790 | sc-166699 AF790 | 200 µg/ml | $350.00 | |||
TCF-4 (D-4) Neutralizing Peptide | sc-166699 P | 100 µg/0.5 ml | $67.00 |