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Quantity: 100 ul                                                                                        Application: WB

Predicted I Observed M.W.: 129 kDa                                                        Uniprot ID: P14713

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Background:

Phytochrome B (PHYB) is a regulatory photoreceptor, which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion. PHYA is involved in the flowering time regulation.

Other Names:

Phytochrome B, HY3

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana PHYB (At2g18790). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

 

Fig 1. A) Western blot analysis of total protein extracts from wild type (WT) or

Myc-tagged PHYB overexpressing (Myc-PHYB) arabidopsis leaves, using anti-PHYB (C1)

(R1244-2) at RT for 2 h. B) Same as in A except that protein extracts(100ug)  were

prepared from 4 day-old, dark-grown WT or phyB-5 null mutant (Mut) arabidopsis seedlings,

using the same Ab at 1:1000 dilution at 4⁰C overnight (data in B provided by W. Hu, UC Davis).