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EID-2 Antibody (C-8): sc-514902
- EID-2 Antibody (C-8) is a mouse monoclonal IgG2a κ EID-2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 110-138 within an internal region of EID-2 of human origin
- EID-2 Antibody (C-8) is recommended for detection of EID-2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-EID-2 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EID-2 (C-8): sc-514902.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for EID-2 Antibody (C-8) for WB applications. These reagents are now offered in bundles with EID-2 Antibody (C-8) (see ordering information below).
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EID-2 Antibody (C-8) is an IgG2a κ mouse monoclonal EID-2 antibody (also designated EID-2 antibody) that detects the EID-2 protein of mouse, rat and human origin by WB, IP, IF and ELISA. EID-2 Antibody (C-8) is available as both the non-conjugated anti-EID-2 antibody form, as well as multiple conjugated forms of anti-EID-2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. As a regulator of transcription via chromatin remodeling, p300 is a histone acetyltransferase that binds to adenovirus E1A protein and may play a role in its transforming capacity. EID-2 (EP300-interacting inhibitor of differentiation 2), also known as CREBBP/EP300 inhibitor 2, is a 236 amino acid nuclear protein that cooperates with EID-2B to bind to the C-terminus of p300 to inhibit its activity. It also represses MYOD-dependent transcription and muscle differentiation. By interacting with SMAD2, SMAD3 and SMAD4, EID-2 selectively blocks the formation of TGFbeta-induced SMAD3-SMAD4 complex, thereby repressing TGFbeta/SMAD3-dependent signaling. Though it is abundantly expressed in placenta, EID-2 is highly expressed in skeletal muscle, heart, liver, brain and kidney. There are two isoforms of EID-2 that are produced as a result of alternative splicing events.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Cells degrade a novel inhibitor of differentiation with E1A-like properties upon exiting the cell cycle. | Miyake, S., et al. 2000. Mol Cell Biol. 20: 8889-902. PMID: 11073989
- A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation. | Miyake, S., et al. 2003. J Biol Chem. 278: 17060-5. PMID: 12586827
- The multifunctional role of E1A in the transcriptional regulation of CREB/CBP-dependent target genes. | Brockmann, D. and Esche, H. 2003. Curr Top Microbiol Immunol. 272: 97-129. PMID: 12747548
- EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD. | Ji, A., et al. 2003. Gene. 318: 35-43. PMID: 14585496
- A novel E1A-like inhibitor of differentiation (EID) family member, EID-2, suppresses transforming growth factor (TGF)-beta signaling by blocking TGF-beta-induced formation of Smad3-Smad4 complexes. | Lee, HJ., et al. 2004. J Biol Chem. 279: 2666-72. PMID: 14612439
- A novel EID family member, EID-3, inhibits differentiation and forms a homodimer or heterodimer with EID-2. | Sasajima, Y., et al. 2005. Biochem Biophys Res Commun. 333: 969-75. PMID: 15970276
- EID3 is a novel EID family member and an inhibitor of CBP-dependent co-activation. | Bαvner, A., et al. 2005. Nucleic Acids Res. 33: 3561-9. PMID: 15987788
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EID-2 Antibody (C-8) | sc-514902 | 200 µg/ml | $310.00 | |||
EID-2 Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-526401 | 200 µg Ab; 10 µg BP | $348.00 | |||
EID-2 Antibody (C-8): m-IgG2a BP-HRP Bundle | sc-546623 | 200 µg Ab; 10 µg BP | $348.00 | |||
EID-2 Antibody (C-8) AC | sc-514902 AC | 500 µg/ml, 25% agarose | $408.00 | |||
EID-2 Antibody (C-8) HRP | sc-514902 HRP | 200 µg/ml | $310.00 | |||
EID-2 Antibody (C-8) FITC | sc-514902 FITC | 200 µg/ml | $324.00 | |||
EID-2 Antibody (C-8) PE | sc-514902 PE | 200 µg/ml | $337.00 | |||
EID-2 Antibody (C-8) Alexa Fluor® 488 | sc-514902 AF488 | 200 µg/ml | $350.00 | |||
EID-2 Antibody (C-8) Alexa Fluor® 546 | sc-514902 AF546 | 200 µg/ml | $350.00 | |||
EID-2 Antibody (C-8) Alexa Fluor® 594 | sc-514902 AF594 | 200 µg/ml | $350.00 | |||
EID-2 Antibody (C-8) Alexa Fluor® 647 | sc-514902 AF647 | 200 µg/ml | $350.00 | |||
EID-2 Antibody (C-8) Alexa Fluor® 680 | sc-514902 AF680 | 200 µg/ml | $350.00 | |||
EID-2 Antibody (C-8) Alexa Fluor® 790 | sc-514902 AF790 | 200 µg/ml | $350.00 | |||
EID-2 (C-8) Neutralizing Peptide | sc-514902 P | 100 µg/0.5 ml | $67.00 |