Atm Antibody (G-12): sc-377293

Atm Antibody (G-12) is an IgG₁ κ mouse monoclonal Atm antibody (also designated ATM serine/threonine kinase antibody) that detects the Atm protein of human origin by WB, IP, IF, IHC(P) and ELISA. Atm Antibody (G-12) is available as both the non-conjugated anti-Atm antibody form, as well as multiple conjugated forms of anti-Atm antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiec-tases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. ATM signaling and genomic stability in response to DNA damage.  |  Lavin, MF., et al. 2005. Mutat Res. 569: 123-32. PMID: 15603757
2. Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM.  |  Hickson, I., et al. 2004. Cancer Res. 64: 9152-9. PMID: 15604286
3. Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma.  |  Liu, A., et al. 2005. Lab Invest. 85: 436-46. PMID: 15696190
4. Ataxia-telangiectasia-mutated dependent phosphorylation of Artemis in response to DNA damage.  |  Chen, L., et al. 2005. Cancer Sci. 96: 134-41. PMID: 15723659
5. PIK-related kinases: DNA repair, recombination, and cell cycle checkpoints.  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
6. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product.  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
7. Discovery of AT gene sparks biomedical research bonanza.  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
8. A single ataxia telangiectasia gene with a product similar to PI-3 kinase.  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Atm Antibody (G-12) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-Atm Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Atm Antibody (G-12) Specifications

Alternate Product Name

ATM

Product Clone Name

G-12

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

2581-2860

Epitope Target

Atm (ataxia telangiectasia protein)

Epitope Species

human

Product Reactivity/Detection

Atm

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

26 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Kappa Light Chain Binding Proteins

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-Atm antibody is p-Atm Antibody (10H11.E12): sc-47739.