p-c-Abl Antibody (7.Tyr 412): sc-293130

p-c-Abl Antibody (7.Tyr 412) is an IgG2a κ mouse monoclonal p-c-Abl antibody (also designated p-c-Abl antibody or ABL1 antibody) that detects Tyr 412 phosphorylated c-Abl of human origin by WB, IP and ELISA. p-c-Abl Antibody (7.Tyr 412) is available as both conjugated and non-conjugated anti-p-c-Abl antibody forms. The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia (CML), 20-25% of adult acute lymphoblastic leukemia (ALL) and 2-5% of pediatric ALL. In these leukemias the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing the Philadelphia (Ph1) chromosome. The molecular consequence of this trans-location is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein-tyrosine kinase. The Bcr gene has been shown to encode a GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, p21rac.

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References:

1. Three-dimensional solution structure of the src homology 2 domain of c-abl.  |  Overduin, M., et al. 1992. Cell. 70: 697-704. PMID: 1505033
2. Bcr encodes a GTPase-activating protein for p21rac.  |  Diekmann, D., et al. 1991. Nature. 351: 400-2. PMID: 1903516
3. Evidence of a new chimeric bcr/c-abl mRNA in patients with chronic myelocytic leukemia and the Philadelphia chromosome.  |  Stam, K., et al. 1985. N Engl J Med. 313: 1429-33. PMID: 3864009
4. Lymphosarcoma: virus-induced thymic-independent disease in mice.  |  Abelson, HT. and Rabstein, LS. 1970. Cancer Res. 30: 2213-22. PMID: 4318922
5. Sequences of the A-MuLV protein needed for fibroblast and lymphoid cell transformation.  |  Prywes, R., et al. 1983. Cell. 34: 569-79. PMID: 6193890
6. An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity.  |  Konopka, JB., et al. 1984. Cell. 37: 1035-42. PMID: 6204766
7. A cellular oncogene is translocated to the Philadelphia chromosome in chronic myelocytic leukaemia.  |  de Klein, A., et al. 1982. Nature. 300: 765-7. PMID: 6960256

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-c-Abl Antibody (7.Tyr 412) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-c-Abl Antibody (7.Tyr 412) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-c-Abl Antibody (7.Tyr 412) Specifications

Alternate Product Name

ABL1

Product Clone Name

7.Tyr 412

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

Tyr 412

Epitope Target

c-Abl

Epitope Species

human

Product Reactivity/Detection

Tyr 412 phosphorylated c-Abl

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

4 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, HRP: Horseradish Peroxidase

The preferred c-Abl antibody is c-Abl Antibody (24-11): sc-23.