CaMKII beta Antibody (D-6): sc-376828

CaMKIIβ Antibody (D-6) is an IgG₁ κ mouse monoclonal CaMKII beta antibody (also designated CAMK2B antibody or calcium/calmodulin dependent protein kinase II beta antibody) that detects the CaMKII beta protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. CaMKIIβ Antibody (D-6) is available as both the non-conjugated anti-CaMKII beta antibody form, as well as multiple conjugated forms of anti-CaMKII beta antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but phosphorylation by a CaMK is also required for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The cardiac sarcoplasmic reticulum phospholamban kinase is a distinct delta-CaM kinase isozyme.  |  Baltas, LG., et al. 1995. FEBS Lett. 373: 71-5. PMID: 7589437
2. Characterization of a Ca2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase.  |  Tokumitsu, H., et al. 1995. J Biol Chem. 270: 19320-4. PMID: 7642608
3. Calcium/calmodulin-dependent protein kinase II downregulates both calcineurin and protein kinase C-mediated pathways for cytokine gene transcription in human T cells.  |  Hama, N., et al. 1995. J Exp Med. 181: 1217-22. PMID: 7869038
4. G1 cell cycle arrest and apoptosis are induced in NIH 3T3 cells by KN-93, an inhibitor of CaMK-II (the multifunctional Ca2+/CaM kinase).  |  Tombes, RM., et al. 1995. Cell Growth Differ. 6: 1063-70. PMID: 8519682
5. Overexpression of Ca2+/calmodulin-dependent protein kinase II inhibits neurite outgrowth of PC12 cells.  |  Tashima, K., et al. 1996. J Neurochem. 66: 57-64. PMID: 8522989
6. Activation of Ca2+/calmodulin-dependent protein kinase (CaM-kinase) IV by CaM-kinase kinase in Jurkat T lymphocytes.  |  Park, IK. and Soderling, TR. 1995. J Biol Chem. 270: 30464-9. PMID: 8530476

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CaMKII beta Antibody (D-6) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CaMKII beta Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CaMKII beta Antibody (D-6) Specifications

Alternate Product Name

CAMK2B

Product Clone Name

D-6

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

511-540

Epitope Target

CaMKIIβ

Epitope Species

mouse

Product Reactivity/Detection

CaMKIIβ and CaMKIIδ

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

canine and bovine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

5 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Kappa Light Chain Binding Proteins

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p-CaMKII antibody is p-CaMKII Antibody (22B1): sc-32289.