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GRK 3 Antibody (C-11): sc-365197
- GRK 3 Antibody (C-11) is a mouse monoclonal IgG1 κ GRK 3 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 646-688 mapping at the C-terminus of GRK 3 of human origin
- GRK 3 Antibody (C-11) is recommended for detection of GRK 3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GRK 3 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GRK 3 (C-11): sc-365197.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GRK 3 Antibody (C-11) for WB applications. These reagents are now offered in bundles with GRK 3 Antibody (C-11) (see ordering information below).
GRK 3 Antibody (C-11) is an IgG1 κ mouse monoclonal GRK 3 antibody (also designated GRK3 antibody, BARK2 antibody, or ADRBK2 antibody) that detects the GRK 3 protein of mouse, rat and human origin by WB, IP, IF and ELISA. GRK 3 Antibody (C-11) is available as both the non-conjugated anti-GRK 3 antibody form, as well as multiple conjugated forms of anti-GRK 3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of β-adrenergic receptor kinase: GRK 2 (βARK, βARK1) and GRK 3 (βARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- The receptor kinase family: primary structure of rhodopsin kinase reveals similarities to the beta-adrenergic receptor kinase. | Lorenz, W., et al. 1991. Proc Natl Acad Sci U S A. 88: 8715-9. PMID: 1656454
- Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family. | Benovic, JL., et al. 1991. J Biol Chem. 266: 14939-46. PMID: 1869533
- Turning off the signal: desensitization of beta-adrenergic receptor function. | Hausdorff, WP., et al. 1990. FASEB J. 4: 2881-9. PMID: 2165947
- Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases. | Premont, RT., et al. 1994. J Biol Chem. 269: 6832-41. PMID: 8120045
- An approach to the study of G-protein-coupled receptor kinases: an in vitro-purified membrane assay reveals differential receptor specificity and regulation by G beta gamma subunits. | Pei, G., et al. 1994. Proc Natl Acad Sci U S A. 91: 3633-6. PMID: 8170959
- Functionally active targeting domain of the beta-adrenergic receptor kinase: an inhibitor of G beta gamma-mediated stimulation of type II adenylyl cyclase. | Inglese, J., et al. 1994. Proc Natl Acad Sci U S A. 91: 3637-41. PMID: 8170960
- Structure and mechanism of the G protein-coupled receptor kinases. | Inglese, J., et al. 1993. J Biol Chem. 268: 23735-8. PMID: 8226899
- Structural basis for receptor subtype-specific regulation revealed by a chimeric beta 3/beta 2-adrenergic receptor. | Liggett, SB., et al. 1993. Proc Natl Acad Sci U S A. 90: 3665-9. PMID: 8386380
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRK 3 Antibody (C-11) | sc-365197 | 200 µg/ml | $310.00 | |||
GRK 3 Antibody (C-11): m-IgG Fc BP-HRP Bundle | sc-529324 | 200 µg Ab; 10 µg BP | $348.00 | |||
GRK 3 Antibody (C-11): m-IgGκ BP-HRP Bundle | sc-522160 | 200 µg Ab, 40 µg BP | $348.00 | |||
GRK 3 Antibody (C-11): m-IgG1 BP-HRP Bundle | sc-543457 | 200 µg Ab; 20 µg BP | $348.00 | |||
GRK 3 Antibody (C-11) AC | sc-365197 AC | 500 µg/ml, 25% agarose | $408.00 | |||
GRK 3 Antibody (C-11) HRP | sc-365197 HRP | 200 µg/ml | $310.00 | |||
GRK 3 Antibody (C-11) FITC | sc-365197 FITC | 200 µg/ml | $324.00 | |||
GRK 3 Antibody (C-11) PE | sc-365197 PE | 200 µg/ml | $337.00 | |||
GRK 3 Antibody (C-11) Alexa Fluor® 488 | sc-365197 AF488 | 200 µg/ml | $350.00 | |||
GRK 3 Antibody (C-11) Alexa Fluor® 546 | sc-365197 AF546 | 200 µg/ml | $350.00 | |||
GRK 3 Antibody (C-11) Alexa Fluor® 594 | sc-365197 AF594 | 200 µg/ml | $350.00 | |||
GRK 3 Antibody (C-11) Alexa Fluor® 647 | sc-365197 AF647 | 200 µg/ml | $350.00 | |||
GRK 3 Antibody (C-11) Alexa Fluor® 680 | sc-365197 AF680 | 200 µg/ml | $350.00 | |||
GRK 3 Antibody (C-11) Alexa Fluor® 790 | sc-365197 AF790 | 200 µg/ml | $350.00 |