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GSK3 beta Antibody (11B9): sc-81462

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Datasheets
  • GSK3 beta Antibody (11B9) is a mouse monoclonal IgG1, cited in 76 publications, provided at 50 µg/0.5 ml
  • raised against the N-terminus of GSK-3β of human origin
  • recommended for detection of GSK-3β of mouse, rat and human origin by WB and IP; also reactive with additional species, including canine
  • See GSK3 alpha/beta (0011-A): sc-7291 for GSK3 beta antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GSK3 beta (11B9): sc-81462.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GSK3 beta Antibody (11B9) for WB applications. These reagents are now offered in bundles with GSK3 beta Antibody (11B9) (see ordering information below).

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GSK-3β Antibody (11B9) is an IgG1 mouse monoclonal GSK-3β antibody (also designated GSK-3β antibody or GSK3 antibody) that detects GSK-3β of mouse, rat and human origin by WB and IP. GSK-3β Antibody (11B9) is available as the non-conjugated anti-GSK-3β antibody form. Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellular adhesion, and has been implicated in Alzheimer′s disease. Two forms of GSK-3, designated GSK-3α and GSK-3β, have been identified and differ in their subcellular localization. Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer′s disease (5,6). Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer′s disease..

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

GSK3 beta Antibody (11B9)

sc-81462
50 µg/0.5 ml
$310.00

GSK3 beta Antibody (11B9): m-IgG Fc BP-HRP Bundle

sc-539613
50 µg Ab; 10 µg BP
$348.00

GSK3 beta Antibody (11B9): m-IgG1 BP-HRP Bundle

sc-541600
50 µg Ab; 20 µg BP
$348.00

What is the recommended dilution to use GSK-3β (11B9): sc-81462 monoclonal antibody in Western blot?

Asked by: cjMara
We recommend using GSK-3β (11B9): sc-81462 monoclonal antibody at a starting dilution of 1:200 (range 1:100-1:1,000) for Western blotting.
Answered by: Technical Support 9
Date published: 2017-01-26
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Rated 5 out of 5 by from Strong stainingThis antibody has a very strong signal used at 1:1000 dilution for western blot. Already tested in both differentiated SHSY-5Y and mice brain tissue samples
Date published: 2022-12-21
Rated 5 out of 5 by from Nice antibodySpecific for gsk3 beta at 1:500. May be even good to use at 1:1000 but we just never tried. This cell line is HEK293.
Date published: 2018-11-28
Rated 5 out of 5 by from strong signalStrong signal observed by WB with GSK-3 beta Antibody (11B9)
Date published: 2017-01-24
Rated 4 out of 5 by from Published data for WB in mouse R390A cPublished data for WB in mouse R390A c-src and D338N c-src cell lines. -SCBT Publication Review
Date published: 2015-05-19
Rated 4 out of 5 by from Worked well in WB in human GSKWorked well in WB in human GSK-3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from Published data for IP and WB in mouse kidneyPublished data for IP and WB in mouse kidney proximal tubular epithelial cells. -SCBT Publication Review
Date published: 2015-04-04
Rated 4 out of 5 by from Published data for WB in rat brainPublished data for WB in rat brain. -SCBT Publication Review
Date published: 2015-01-10
Rated 4 out of 5 by from Clear band in WB in mouse GSKClear band in WB in mouse GSK-3 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2014-02-20
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GSK3 beta Antibody (11B9) is rated 4.4 out of 5 by 8.
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