HXK II Antibody (1A7): sc-130358

HXK II Antibody (1A7) is an IgG1 κ mouse monoclonal HXK II antibody that detects HXK II of mouse, rat and human origin by WB, IP and ELISA. HXK II Antibody (1A7) is available as the non-conjugated anti-HXK II antibody form. The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the "set point" for insulin secretion.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HXK II Antibody (1A7) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-HXK II Antibody (1A7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HXK II Antibody (1A7) Specifications

Alternate Product Name

HXK II

Product Clone Name

1A7

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

Epitope Target

HXK II

Epitope Species

human

Product Reactivity/Detection

HXK II

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, ELISA

Vialing Information

Each vial contains 50 µg IgG₁ kappa light chain in 500 µl of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations

21 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

N/A

The preferred HXK II antibody is HXK II Antibody (B-8): sc-374091.