IFN-γ/Interferon gamma Antibody (E-10): sc-373727

IFN-γ Antibody (E-10) is an IgG_2a κ mouse monoclonal IFN-gamma antibody (also designated IFNG antibody, Interferon gamma antibody, Immune interferon antibody, IFN gamma antibody or Interferon-γ antibody) that detects the IFN-gamma protein of human origin by WB, IP, IF and ELISA. IFN-γ Antibody (E-10) is available as both the non-conjugated anti-IFN-gamma antibody form, as well as multiple conjugated forms of anti-IFN-gamma antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Interferon (IFN)-γ is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-γ production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-γ is inducible by IL-2, FGF and EGF. The active form of IFN-γ is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-γ is stabilized by non-covalent interactions through the interface of the helices. IFN-γ translated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IFN-γ/Interferon gamma Antibody (E-10) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-IFN-γ/Interferon gamma Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IFN-γ/Interferon gamma Antibody (E-10) Specifications

Alternate Product Name

IFN-γ

Product Clone Name

E-10

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

25-50

Epitope Target

IFN-γ

Epitope Species

human

Product Reactivity/Detection

precursor and mature IFN-γ

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

13 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Kappa Light Chain Binding Proteins

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred IFN-γ/Interferon gamma antibody is IFN-γ/Interferon gamma Antibody (G-23): sc-8423.