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IFN-γ/Interferon gamma Antibody (G-23): sc-8423
- IFN-γ/Interferon gamma Antibody (G-23) is a mouse monoclonal IgG1 κ, cited in 12 publications, provided at 200 µg/ml
- raised against amino acids 22-166 representing mature interferon-γ (IFN-γ) of human origin
- IFN-γ/Interferon gamma Antibody (G-23) is recommended for detection of precursor and mature IFN-γ of human origin by WB, IP, IF, FCM and ELISA
- Anti-IFN-γ/Interferon gamma Antibody (G-23) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFN-γ/Interferon gamma (G-23): sc-8423.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for IFN-γ/Interferon gamma Antibody (G-23) for WB applications. These reagents are now offered in bundles with IFN-γ/Interferon gamma Antibody (G-23) (see ordering information below).
IFN-γ/Interferon gamma Antibody (G-23) is an IgG1 κ mouse monoclonal IFN-γ antibody that detects precursor and mature IFN-γ of human origin by WB, IP, IF, FCM and ELISA. IFN-γ/Interferon gamma Antibody (G-23) is available as the non-conjugated anti-IFN-γ antibody form, as well as multiple conjugated forms of anti-IFN-γ antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Interferon (IFN)-γ is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-γ production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-γ is inducible by IL-2, FGF and EGF. The active form of IFN-γ is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-γ is stabilized by non-covalent interactions through the interface of the helices. IFN-γ translated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- The antiviral response to gamma interferon. | Costa-Pereira, AP., et al. 2002. J Virol. 76: 9060-8. PMID: 12186889
- Histone deacetylase 1/mSin3A disrupts gamma interferon-induced CIITA function and major histocompatibility complex class II enhanceosome formation. | Zika, E., et al. 2003. Mol Cell Biol. 23: 3091-102. PMID: 12697811
- Interferon-gamma: an overview of signals, mechanisms and functions. | Schroder, K., et al. 2004. J Leukoc Biol. 75: 163-89. PMID: 14525967
- Interferon-gamma activation of polymorphonuclear neutrophil function. | Ellis, TN. and Beaman, BL. 2004. Immunology. 112: 2-12. PMID: 15096178
- Inhibitor of kappaB kinase is required to activate a subset of interferon gamma-stimulated genes. | Sizemore, N., et al. 2004. Proc Natl Acad Sci U S A. 101: 7994-8. PMID: 15148408
- Interferon-gamma-dependent tyrosine phosphorylation of MEKK4 via Pyk2 is regulated by annexin II and SHP2 in keratinocytes. | Halfter, UM., et al. 2005. Biochem J. 388: 17-28. PMID: 15601262
- Role of interferon-gamma in immune cell regulation. | Young, HA. and Hardy, KJ. 1995. J Leukoc Biol. 58: 373-81. PMID: 7561512
- Overview of interleukin-18: more than an interferon-gamma inducing factor. | Dinarello, CA., et al. 1998. J Leukoc Biol. 63: 658-64. PMID: 9620656
- Regulation of interferon-gamma production by IL-12 and IL-18. | Okamura, H., et al. 1998. Curr Opin Immunol. 10: 259-64. PMID: 9638361
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFN-γ/Interferon gamma Antibody (G-23) | sc-8423 | 200 µg/ml | $310.00 | |||
IFN-γ/Interferon gamma Antibody (G-23): m-IgG Fc BP-HRP Bundle | sc-526523 | 200 µg Ab; 10 µg BP | $348.00 | |||
IFN-γ/Interferon gamma Antibody (G-23): m-IgG1 BP-HRP Bundle | sc-531896 | 200 µg Ab; 20 µg BP | $348.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) AC | sc-8423 AC | 500 µg/ml, 25% agarose | $408.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) HRP | sc-8423 HRP | 200 µg/ml | $310.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) FITC | sc-8423 FITC | 200 µg/ml | $324.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) PE | sc-8423 PE | 200 µg/ml | $337.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) Alexa Fluor® 488 | sc-8423 AF488 | 200 µg/ml | $350.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) Alexa Fluor® 546 | sc-8423 AF546 | 200 µg/ml | $350.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) Alexa Fluor® 594 | sc-8423 AF594 | 200 µg/ml | $350.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) Alexa Fluor® 647 | sc-8423 AF647 | 200 µg/ml | $350.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) Alexa Fluor® 680 | sc-8423 AF680 | 200 µg/ml | $350.00 | |||
IFN-γ/Interferon gamma Antibody (G-23) Alexa Fluor® 790 | sc-8423 AF790 | 200 µg/ml | $350.00 |