Pdcd-1L2 Antibody (TY25): sc-57398

Pdcd-1L2 Antibody (TY25) is an IgG_2a rat monoclonal Pdcd-1L2 antibody (also designated Pdcd-1L2 antibody) that detects the Pdcd-1L2 protein of mouse origin by WB, IP, IF, IHC(P) and FCM. Pdcd-1L2 Antibody (TY25) is available as both the non-conjugated anti-Pdcd-1L2 antibody form, as well as multiple conjugated forms of anti-Pdcd-1L2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells, and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. Pdcd-1L1 (programmed cell death ligand-1), also known as B7-H1 or PD-L1, is 290 amino acid type I transmembrane protein which is 20% and 15% identical to B7-1 and B7-2, respectively. Pdcd-1L2 has immunoglobulin V-like and C-like domains and a 30 amino acid cytoplasmic tail. It does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of Pdcd-1L1 co-stimulation. The gene which encodes Pdcd-1L1 maps to human chromosome 9p24. Pdcd-1L2 (programmed cell death ligand-2) is a 73 amino acid protein which contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The gene which encodes Pdcd-1L2 maps to human chromosome 9p24.2. The constitutive expression of Pdcd-1L1 and Pdcd-1L2 on paren-chymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion.  |  Dong, H., et al. 1999. Nat Med. 5: 1365-9. PMID: 10581077
2. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation.  |  Freeman, GJ., et al. 2000. J Exp Med. 192: 1027-34. PMID: 11015443
3. PD-L2 is a second ligand for PD-1 and inhibits T cell activation.  |  Latchman, Y., et al. 2001. Nat Immunol. 2: 261-8. PMID: 11224527
4. PD-1: an inhibitory immunoreceptor involved in peripheral tolerance.  |  Nishimura, H. and Honjo, T. 2001. Trends Immunol. 22: 265-8. PMID: 11323285

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pdcd-1L2 Antibody (TY25) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Pdcd-1L2 Antibody (TY25) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pdcd-1L2 Antibody (TY25) Specifications

Alternate Product Name

Pdcd-1L2

Product Clone Name

TY25

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a

Amino Acids

Epitope Target

Pdcd-1L2

Epitope Species

mouse

Product Reactivity/Detection

Pdcd-1L2

Species Reactivity/Detection

mouse

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), FCM

Vialing Information

Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

1 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin