Flk-1/KDR/VEGFR2 Antibody (A-3): sc-6251

Flk-1/KDR/VEGFR2 Antibody (A-3) is an IgG1 κ mouse monoclonal VEGFR2 antibody that detects VEGFR2 of mouse, rat and human origin by WB, IP, IF and IHC(P). Flk-1/KDR/VEGFR2 Antibody (A-3) is available as the non-conjugated anti-VEGFR2 antibody form, as well as multiple conjugated forms of anti-VEGFR2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1) (1-3), Flk-1 (also designated VEGF-R2) (4-6) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs) (3,5). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The fms-like tyrosine kinase, a receptor for vascular endothelial growth factor.  |  de Vries, C., et al. 1992. Science. 255: 989-91. PMID: 1312256
2. A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit.  |  Matthews, W., et al. 1991. Proc Natl Acad Sci U S A. 88: 9026-30. PMID: 1717995
3. Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family.  |  Shibuya, M., et al. 1990. Oncogene. 5: 519-24. PMID: 2158038
4. High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis.  |  Millauer, B., et al. 1993. Cell. 72: 835-46. PMID: 7681362
5. Vascular endothelial growth factor receptor expression during embryogenesis and tissue repair suggests a role in endothelial differentiation and blood vessel growth.  |  Peters, KG., et al. 1993. Proc Natl Acad Sci U S A. 90: 8915-9. PMID: 7692439
6. The FLT4 gene encodes a transmembrane tyrosine kinase related to the vascular endothelial growth factor receptor.  |  Galland, F., et al. 1993. Oncogene. 8: 1233-40. PMID: 8386825
7. NYK/FLK-1: a putative receptor protein tyrosine kinase isolated from E10 embryonic neuroepithelium is expressed in endothelial cells of the developing embryo.  |  Oelrichs, RB., et al. 1993. Oncogene. 8: 11-8. PMID: 8423988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Flk-1/KDR/VEGFR2 Antibody (A-3) has 565 citations in a variety of scientific publications.

Primary antibodies like Anti-Flk-1/KDR/VEGFR2 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Flk-1/KDR/VEGFR2 Antibody (A-3) Specifications

Alternate Product Name

VEGFR2

Product Clone Name

A-3

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

1158-1345

Epitope Target

VEGFR2

Epitope Species

mouse

Product Reactivity/Detection

VEGFR2

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P)

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

565 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent