SMO/Smoothened Antibody (E-5): sc-166685

Smo Antibody (E-5) is an IgG_2a κ mouse monoclonal Smo antibody (also designated Smoothened antibody) that detects the Smo protein of mouse, rat and human origin by WB, IP, IF and ELISA. Smo Antibody (E-5) is available as both the non-conjugated anti-Smo antibody form, as well as multiple conjugated forms of anti-Smo antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repessor opposing the effects of the hedgehog. Smoothened (Smo), a seven transmembrane receptor, is complexed with patched in many tissues and is believed to be an essential component in the Hh signaling pathway.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Identification of an amplified, highly expressed gene in a human glioma.  |  Kinzler, KW., et al. 1987. Science. 236: 70-3. PMID: 3563490
2. Mutations affecting segment number and polarity in Drosophila.  |  Nυsslein-Volhard, C. and Wieschaus, E. 1980. Nature. 287: 795-801. PMID: 6776413
3. Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis.  |  Roelink, H., et al. 1995. Cell. 81: 445-55. PMID: 7736596
4. Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types in CNS explants.  |  Martí, E., et al. 1995. Nature. 375: 322-5. PMID: 7753196
5. Activity of Wnt-1 as a transmembrane protein.  |  Parkin, NT., et al. 1993. Genes Dev. 7: 2181-93. PMID: 8224845
6. Human homolog of patched, a candidate gene for the basal cell nevus syndrome.  |  Johnson, RL., et al. 1996. Science. 272: 1668-71. PMID: 8658145
7. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.  |  Stone, DM., et al. 1996. Nature. 384: 129-34. PMID: 8906787

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMO/Smoothened Antibody (E-5) has 92 citations in a variety of scientific publications.

Primary antibodies like Anti-SMO/Smoothened Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMO/Smoothened Antibody (E-5) Specifications

Alternate Product Name

Smoothened

Product Clone Name

E-5

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

488-787

Epitope Target

Smo

Epitope Species

human

Product Reactivity/Detection

Smo

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

92 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG_2a Binding Proteins

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin