Adenylate cyclase 8/AC8/ADCY8 Antibody (B-6): sc-377323

A cyclase VIII Antibody (B-6) is an IgG₁ κ mouse monoclonal A cyclase VIII antibody (also designated A cyclase VIII antibody) that detects the A cyclase VIII protein of mouse, rat and human origin by WB, IP, IF and ELISA. A cyclase VIII Antibody (B-6) is available as both the non-conjugated anti-A cyclase VIII antibody form, as well as multiple conjugated forms of anti-A cyclase VIII antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Ga s) for GTP, dissociation of GTP-Gα s from Gβ γ and Gα s-mediated activation of adenylyl cyclase. Adenylyl cyclase type VIII (A cyclase VIII) is one of the three mammalian calcium-stimulated isoforms, each of which is expressed in a region-specific manner in the central nervous system. In addition to the high expression in the brain, A cyclase VIII is also expressed in the lung. Ca2+/calmodulin-dependent A cyclase VIII immunoreactivity is increased in alcoholic corpus amyadaloideum and hippocampus, suggesting that adenyl cyclase may play a role in the pathophysiology of alcoholism. A significant decrease in the level of A cyclase I and a tendency to decrease in the level of A cyclase VIII in Alzheimer′s disease hippocampus suggests that A cyclase I and VIII may play an essential role in learning and memory. A cyclase VIII knock-out mice do not have normal increases in behavioral markers of anxiety; thus, A cyclase VIII may also function in the modulation of anxiety.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The 5′-flanking region of the mouse adenylyl cyclase type VIII gene imparts tissue-specific expression in transgenic mice.  |  Muglia, LM., et al. 1999. J Neurosci. 19: 2051-8. PMID: 10066258
2. Altered stress-induced anxiety in adenylyl cyclase type VIII-deficient mice.  |  Schaefer, ML., et al. 2000. J Neurosci. 20: 4809-20. PMID: 10864938
3. Hippocampal level of neural specific adenylyl cyclase type I is decreased in Alzheimer′s disease.  |  Yamamoto, M., et al. 2000. Biochim Biophys Acta. 1535: 60-8. PMID: 11113632
4. Increased levels of calcium-sensitive adenylyl cyclase subtypes in the limbic system of alcoholics: evidence for a specific role of cAMP signaling in the human addictive brain.  |  Yamamoto, M., et al. 2001. Brain Res. 895: 233-7. PMID: 11259782
5. Adenylyl cyclases.  |  Tang, WJ. and Gilman, AG. 1992. Cell. 70: 869-72. PMID: 1525824
6. The GTPase superfamily: a conserved switch for diverse cell functions.  |  Bourne, HR., et al. 1990. Nature. 348: 125-32. PMID: 2122258
7. G proteins: transducers of receptor-generated signals.  |  Gilman, AG. 1987. Annu Rev Biochem. 56: 615-49. PMID: 3113327
8. Distinct patterns of bidirectional regulation of mammalian adenylyl cyclases.  |  Taussig, R., et al. 1994. J Biol Chem. 269: 6093-100. PMID: 8119955

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Adenylate cyclase 8/AC8/ADCY8 Antibody (B-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Adenylate cyclase 8/AC8/ADCY8 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Adenylate cyclase 8/AC8/ADCY8 Antibody (B-6) Specifications

Alternate Product Name

A cyclase VIII

Product Clone Name

B-6

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

561-830

Epitope Target

A cyclase VIII

Epitope Species

human

Product Reactivity/Detection

A cyclase VIII

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

equine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

0 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin