Acid Ceramidase Antibody (23): sc-136275

Acid Ceramidase Antibody (23) is an IgG₁ κ mouse monoclonal Acid Ceramidase antibody (also designated ASAH1 antibody, N-Acylsphingosine Amidohydrolase 1 antibody, Acylsphingosine Deacylase antibody, Acid Ceramidase antibody, ACDase antibody, PHP32 antibody, AC antibody, N-Acylethanolamine Hydrolase ASAH1 antibody, Putative 32 KDa Heart Protein antibody, Acid CDase antibody, ASAH antibody, or N-Acylsphingosine Amidohydrolase antibody) that detects the Acid Ceramidase protein of human origin by . Acid Ceramidase Antibody (23) is available as the non-conjugated anti-Acid Ceramidase antibody. Acid ceramidase catalyzes the degradation of ceramide in normal tissues, and deficiency leads to accumulation of ceramide in tissues, a hallmark of Farber disease. Effected individuals experience early onset joint problems and neurological problems, owing to mutations in the acid ceramidase gene. Bioinformatic analysis of gene expression also reveals acid ceramidase to be among the 5 most important genes associated with melanoma. In addition to ceramide hydrolysis, purified acid ceramidase also exhibits the ability to catalyze ceramide synthesis, utilizing [14C]lauric acid and sphingosine as substrates. Interestingly, pH regulates which reaction is favored; for hydrolysis the pH optimum is 4.5, whereas for the reverse reaction favors a pH of 5.5, further supporting a complex and central role for acid ceramidase in sphingolipid metabolism.

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References:

1. Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death.  |  Strelow, A., et al. 2000. J Exp Med. 192: 601-12. PMID: 10974027
2. Interfacial regulation of acid ceramidase activity. Stimulation of ceramide degradation by lysosomal lipids and sphingolipid activator proteins.  |  Linke, T., et al. 2001. J Biol Chem. 276: 5760-8. PMID: 11104761
3. Human acid ceramidase: processing, glycosylation, and lysosomal targeting.  |  Ferlinz, K., et al. 2001. J Biol Chem. 276: 35352-60. PMID: 11451951
4. A bioinformatic approach to the identification of candidate genes for the development of new cancer diagnostics.  |  Musumarra, G., et al. 2003. Biol Chem. 384: 321-7. PMID: 12675527
5. The reverse activity of human acid ceramidase.  |  Okino, N., et al. 2003. J Biol Chem. 278: 29948-53. PMID: 12764132
6. Purification and characterization of recombinant, human acid ceramidase. Catalytic reactions and interactions with acid sphingomyelinase.  |  He, X., et al. 2003. J Biol Chem. 278: 32978-86. PMID: 12815059
7. Elevation of the level and activity of acid ceramidase in Alzheimer′s disease brain.  |  Huang, Y., et al. 2004. Eur J Neurosci. 20: 3489-97. PMID: 15610181
8. Molecular cloning and characterization of a full-length complementary DNA encoding human acid ceramidase. Identification Of the first molecular lesion causing Farber disease.  |  Koch, J., et al. 1996. J Biol Chem. 271: 33110-5. PMID: 8955159
9. Purification, characterization, and biosynthesis of human acid ceramidase.  |  Bernardo, K., et al. 1995. J Biol Chem. 270: 11098-102. PMID: 7744740

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Acid Ceramidase Antibody (23) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Acid Ceramidase Antibody (23) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Acid Ceramidase Antibody (23) Specifications

Alternate Product Name

ASAH1

Product Clone Name

23

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

88-182

Epitope Target

Acid Ceramidase α

Epitope Species

human

Product Reactivity/Detection

Acid Ceramidase α

Species Reactivity/Detection

human

Additional Mammalian Reactivity

canine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

4 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

N/A

The preferred Acid Ceramidase antibody is Acid Ceramidase Antibody (F-9): sc-518165.