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α-2M Antibody (H-8): sc-390544
- α-2M Antibody (H-8) is a mouse monoclonal IgG1 κ α-2M antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 413-469 mapping within an internal region of α-2M of human origin
- alpha-2M Antibody (H-8) is recommended for detection of α-2M of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-alpha-2M Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α-2M (H-8): sc-390544.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for α-2M Antibody (H-8) for WB and IHC(P) applications. These reagents are now offered in bundles with α-2M Antibody (H-8) (see ordering information below).
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α-2M Antibody (H-8) is an IgG1 κ mouse monoclonal alpha-2M antibody (also designated α-2M antibody) that detects the alpha-2M protein of human origin by WB, IP, IF, IHC(P) and ELISA. α-2M Antibody (H-8) is available as both the non-conjugated anti-alpha-2M antibody form, as well as multiple conjugated forms of anti-alpha-2M antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. α-2-Macroglobulin (α-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, α-2M was characterized as a pan-proteinase inhibitor that was able to "bait" proteinases into cleaving specific peptide sequences on α-2M. This interaction induces a conformational change in α-2M, thus enabling it to "trap" the proteinase and further inhibit its activity. Subsequently, α-2M has been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor β (TGFβ) in serum is primarily bound to α-2M, which renders TGFβ inactive. α-2M also binds to IL-6 and, thereby, increases the concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved in mediating the inflammatory cascade. Mutations and deletions in the gene encoding α-2M are associated with an increased incidence of Alzheimer′s Disease (AD), which is consistent with the role of α-2M in mediating the clearance and degradation of A β, the major component of β-Amyloid deposits accumulated during AD.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Cloning of the human alpha 2-macroglobulin gene and detection of mutations in two functional domains: the bait region and the thiolester site. | Poller, W., et al. 1992. Hum Genet. 88: 313-9. PMID: 1370808
- Binding of IL-1 beta to alpha-macroglobulins and release by thioredoxin. | Borth, W., et al. 1990. J Immunol. 145: 3747-54. PMID: 1700994
- Sequence analysis of the putative regulatory region of rat alpha 2-macroglobulin gene. | Tsuchiya, Y., et al. 1987. Gene. 57: 73-80. PMID: 2448189
- The interaction of alpha 2-macroglobulin with proteinases. Characteristics and specificity of the reaction, and a hypothesis concerning its molecular mechanism. | Barrett, AJ. and Starkey, PM. 1973. Biochem J. 133: 709-24. PMID: 4201304
- Localization of the binding site for transforming growth factor-beta in human alpha2-macroglobulin to a 20-kDa peptide that also contains the bait region. | Webb, DJ., et al. 1998. J Biol Chem. 273: 13339-46. PMID: 9582381
- Alpha-2 macroglobulin is genetically associated with Alzheimer disease. | Blacker, D., et al. 1998. Nat Genet. 19: 357-60. PMID: 9697696
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α-2M Antibody (H-8) | sc-390544 | 200 µg/ml | $310.00 | |||
α-2M Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-530184 | 200 µg Ab; 10 µg BP | $348.00 | |||
α-2M Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-523577 | 200 µg Ab, 40 µg BP | $348.00 | |||
α-2M Antibody (H-8): m-IgG1 BP-HRP Bundle | sc-543911 | 200 µg Ab; 20 µg BP | $348.00 | |||
α-2M Antibody (H-8) AC | sc-390544 AC | 500 µg/ml, 25% agarose | $408.00 | |||
α-2M Antibody (H-8) HRP | sc-390544 HRP | 200 µg/ml | $310.00 | |||
α-2M Antibody (H-8) FITC | sc-390544 FITC | 200 µg/ml | $324.00 | |||
α-2M Antibody (H-8) PE | sc-390544 PE | 200 µg/ml | $337.00 | |||
α-2M Antibody (H-8) Alexa Fluor® 488 | sc-390544 AF488 | 200 µg/ml | $350.00 | |||
α-2M Antibody (H-8) Alexa Fluor® 546 | sc-390544 AF546 | 200 µg/ml | $350.00 | |||
α-2M Antibody (H-8) Alexa Fluor® 594 | sc-390544 AF594 | 200 µg/ml | $350.00 | |||
α-2M Antibody (H-8) Alexa Fluor® 647 | sc-390544 AF647 | 200 µg/ml | $350.00 | |||
α-2M Antibody (H-8) Alexa Fluor® 680 | sc-390544 AF680 | 200 µg/ml | $350.00 | |||
α-2M Antibody (H-8) Alexa Fluor® 790 | sc-390544 AF790 | 200 µg/ml | $350.00 |