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Aminoacylase-1 Antibody (B-1): sc-271019
- Aminoacylase-1 Antibody (B-1) is a mouse monoclonal IgG1 κ Aminoacylase-1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 265-408 mapping at the C-terminus of Aminoacylase-1 of human origin
- Aminoacylase-1 Antibody (B-1) is recommended for detection of Aminoacylase-1 of human origin by WB, IP, IF and ELISA
- Anti-Aminoacylase-1 Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Aminoacylase-1 (B-1): sc-271019.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Aminoacylase-1 Antibody (B-1) for WB applications. These reagents are now offered in bundles with Aminoacylase-1 Antibody (B-1) (see ordering information below).
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SEE ALSO...
Aminoacylase-1 Antibody (B-1) is an IgG1 κ mouse monoclonal Aminoacylase-1 antibody (also designated Aminoacylase-1 antibody) that detects the Aminoacylase-1 protein of human origin by WB, IP, IF and ELISA. Aminoacylase-1 Antibody (B-1) is available as both the non-conjugated anti-Aminoacylase-1 antibody form, as well as multiple conjugated forms of anti-Aminoacylase-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Aminoacylase-1, also designated N-acyl-L-amino-acid amidohydrolase or ACY-1, is a member of the largest metallopeptidase family called M20A. Aminoacylase-1 is a zinc-binding homodimeric enzyme expressed in kidney, brain, placenta and spleen. It is the most abundant of the aminoacylases. Aminoacylase-1 localizes to the cytoplasm and catalyzes the hydrolysis of N-acylated or N-acetylated amino acids. In addition, Aminoacylase-1 is a sphingosine kinase 1 (SphK1)-interacting protein and may also play a role in regulating responses of the cell to oxidative stress. The gene encoding Aminoacylase-1 is evolutionarily conserved in fish, frog, mouse, rat and human. Deficiency in the Aminoacylase-1 protein may result in defects of brain metabolism and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Mutational analysis of two PWW sequence motifs in human aminoacylase 1. | Lindner, H., et al. 2000. Biol Chem. 381: 1055-61. PMID: 11154063
- Essential roles of zinc ligation and enzyme dimerization for catalysis in the aminoacylase-1/M20 family. | Lindner, HA., et al. 2003. J Biol Chem. 278: 44496-504. PMID: 12933810
- Aminoacylase 1 is a sphingosine kinase 1-interacting protein. | Maceyka, M., et al. 2004. FEBS Lett. 568: 30-4. PMID: 15196915
- Roles of dimerization domain residues in binding and catalysis by aminoacylase-1. | Lindner, HA., et al. 2005. Biochemistry. 44: 15645-51. PMID: 16313167
- Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism. | Sass, JO., et al. 2006. Am J Hum Genet. 78: 401-9. PMID: 16465618
- Probing the catalytic center of porcine aminoacylase 1 by site-directed mutagenesis, homology modeling and substrate docking. | Liu, Z., et al. 2006. J Biochem. 139: 421-30. PMID: 16567407
- NMR spectroscopy of aminoacylase 1 deficiency, a novel inborn error of metabolism. | Engelke, UF., et al. 2008. NMR Biomed. 21: 138-47. PMID: 17516490
- Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. | Cook, RM., et al. 1993. J Biol Chem. 268: 17010-7. PMID: 8394326
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aminoacylase-1 Antibody (B-1) | sc-271019 | 200 µg/ml | $310.00 | |||
Aminoacylase-1 Antibody (B-1): m-IgG Fc BP-HRP Bundle | sc-529098 | 200 µg Ab; 10 µg BP | $348.00 | |||
Aminoacylase-1 Antibody (B-1): m-IgGκ BP-HRP Bundle | sc-521817 | 200 µg Ab, 40 µg BP | $348.00 | |||
Aminoacylase-1 Antibody (B-1): m-IgG1 BP-HRP Bundle | sc-543317 | 200 µg Ab; 20 µg BP | $348.00 | |||
Aminoacylase-1 Antibody (B-1) AC | sc-271019 AC | 500 µg/ml, 25% agarose | $408.00 | |||
Aminoacylase-1 Antibody (B-1) HRP | sc-271019 HRP | 200 µg/ml | $310.00 | |||
Aminoacylase-1 Antibody (B-1) FITC | sc-271019 FITC | 200 µg/ml | $324.00 | |||
Aminoacylase-1 Antibody (B-1) PE | sc-271019 PE | 200 µg/ml | $337.00 | |||
Aminoacylase-1 Antibody (B-1) Alexa Fluor® 488 | sc-271019 AF488 | 200 µg/ml | $350.00 | |||
Aminoacylase-1 Antibody (B-1) Alexa Fluor® 546 | sc-271019 AF546 | 200 µg/ml | $350.00 | |||
Aminoacylase-1 Antibody (B-1) Alexa Fluor® 594 | sc-271019 AF594 | 200 µg/ml | $350.00 | |||
Aminoacylase-1 Antibody (B-1) Alexa Fluor® 647 | sc-271019 AF647 | 200 µg/ml | $350.00 | |||
Aminoacylase-1 Antibody (B-1) Alexa Fluor® 680 | sc-271019 AF680 | 200 µg/ml | $350.00 | |||
Aminoacylase-1 Antibody (B-1) Alexa Fluor® 790 | sc-271019 AF790 | 200 µg/ml | $350.00 |