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Antibodies > MYD88 > LS-C344009
 
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Catalog Number Size Price
LS-C344009-10 10 µg $318 
LS-C344009-100 100 µg (0.5 mg/ml) $470 
MYD88 Antibody - MyD88 antibody IHC-paraffin: Mouse Spleen Tissue.
MYD88 Antibody - MyD88 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
MYD88 Antibody - MyD88 antibody IHC-paraffin: Rat Lung Tissue.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human colon cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human colon cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human mammary cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - igure 10.IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human mammary cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - Western blot analysis of MYD88 using anti-MYD88 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Cardiac Muscle Tissue Lysate Lane 2: HELA Whole Cell Lysate Lane 3: MCF Whole Cell Lysate Lane 4: HEPG2 Whole Cell Lysate Lane 5: JURKAT Whole Cell Lysate Lane 6: RAJI Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYD88 at approximately 33KD. The expected band size for MYD88 is at 33KD.
MYD88 Antibody - MyD88 antibody Western blot. All lanes: Anti MYD88 at 0.5 ug/ml. WB: Recombinant Human MYD88 Protein 0.5ng. Predicted band size: 49 kD. Observed band size: 49 kD.
MYD88 Antibody - Flow Cytometry analysis of A549 cells using anti-MYD88 antibody. Overlay histogram showing A549 cells stained with anti-MYD88 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYD88 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MYD88 Antibody - MyD88 antibody IHC-paraffin: Mouse Spleen Tissue.
MYD88 Antibody - MyD88 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
MYD88 Antibody - MyD88 antibody IHC-paraffin: Rat Lung Tissue.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human colon cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human colon cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human mammary cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - igure 10.IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human mammary cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - IF analysis of MYD88 using anti-MYD88 antibody MYD88 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-MYD88 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYD88 Antibody - Western blot analysis of MYD88 using anti-MYD88 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Cardiac Muscle Tissue Lysate Lane 2: HELA Whole Cell Lysate Lane 3: MCF Whole Cell Lysate Lane 4: HEPG2 Whole Cell Lysate Lane 5: JURKAT Whole Cell Lysate Lane 6: RAJI Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYD88 at approximately 33KD. The expected band size for MYD88 is at 33KD.
MYD88 Antibody - MyD88 antibody Western blot. All lanes: Anti MYD88 at 0.5 ug/ml. WB: Recombinant Human MYD88 Protein 0.5ng. Predicted band size: 49 kD. Observed band size: 49 kD.
MYD88 Antibody - Flow Cytometry analysis of A549 cells using anti-MYD88 antibody. Overlay histogram showing A549 cells stained with anti-MYD88 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYD88 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human MYD88 Antibody (aa44‑264, IHC, WB) LS‑C344009

Polyclonal Rabbit anti‑Human MYD88 Antibody (aa44‑264, IHC, WB) LS‑C344009

Submit Review LSBio Guarantee
Antibody:
MYD88 Rabbit anti-Human Polyclonal (aa44-264) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C344009-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MYD88 Rabbit anti-Human Polyclonal (aa44-264) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MYD88 antibody LS-C344009 is an unconjugated rabbit polyclonal antibody to MYD88 (aa44-264) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human MYD88
Synonyms
MYD88 | MYD88D
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human MyD88 recombinant protein (Position: A44-F264). Human MyD88 shares 84% and 83% amino acid (aa) sequences identity with mouse and rat MyD88, respectively.
Epitope
aa44-264
Specificity
Ubiquitous.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MYD88
Q99836 NM_002468 NP_002459.2

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Requested From: United States
Date Requested: 5/20/2024
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