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Annexin V Antibody (H-3): sc-74438
- Annexin V Antibody (H-3) is a mouse monoclonal IgG2a κ Annexin V antibody, cited in 31 publications, provided at 200 µg/ml
- raised against amino acids 1-319 representing full length Annexin V of human origin
- Annexin V Antibody (H-3) is recommended for detection of Annexin V of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Annexin V Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Annexin V (H-3): sc-74438.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Annexin V Antibody (H-3) for WB applications. These reagents are now offered in bundles with Annexin V Antibody (H-3) (see ordering information below).
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Annexin V Antibody (H-3) is an IgG2a κ mouse monoclonal Annexin V antibody (also designated ANXA5 antibody, Annexin A5 antibody, Endonexin II antibody, Lipocortin V antibody, Calphobindin I antibody or Placental anticoagulant protein 4 antibody) that detects the Annexin V protein of mouse, rat and human origin by WB, IP, IF and ELISA. Annexin V Antibody (H-3) is available as both the non-conjugated anti-Annexin V antibody form, as well as multiple conjugated forms of anti-Annexin V antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain, which binds phospholipids in a Ca2+-dependent manner, and a unique amino-term-inal region, which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino-terminus with possible Ca2+ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl-flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in tissue culture, while Annexin VIII exhibits a more limited distribution. Where co-expressed in the same tissues, Annexin VIII is often expressed at a 100-fold lower level than Annexin V. However, Annexin VIII is preferentially expressed in acute promyelocytic leukemia (APL) cells, which may relate to its role in hematopoietic cell differentiation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Inhibitory effect of annexin V on protein kinase C activity in mesangial cell lysates. | Rothhut, B., et al. 1995. Eur J Biochem. 232: 865-72. PMID: 7588728
- Annexin IV inhibits calmodulin-dependent protein kinase II-activated chloride conductance. A novel mechanism for ion channel regulation. | Chan, HC., et al. 1994. J Biol Chem. 269: 32464-8. PMID: 7798247
- Differential tissue expression of Annexin VIII in human. | Reutelingsperger, CP., et al. 1994. FEBS Lett. 349: 120-4. PMID: 8045287
- Expression of the annexin VIII gene in acute promyelocytic leukemia. | Liu, JH., et al. 1994. Leuk Lymphoma. 13: 381-6. PMID: 8069182
- Structural evolution of the annexin supergene family. | Smith, PD. and Moss, SE. 1994. Trends Genet. 10: 241-6. PMID: 8091504
- Calcium-dependent binding of S100C to the N-terminal domain of annexin I. | Mailliard, WS., et al. 1996. J Biol Chem. 271: 719-25. PMID: 8557678
- Structural and functional characterisation of the voltage sensor in the ion channel human annexin V. | Liemann, S., et al. 1996. J Mol Biol. 258: 555-61. PMID: 8636991
- The high-resolution crystal structure of human annexin III shows subtle differences with annexin V. | Favier-Perron, B., et al. 1996. Biochemistry. 35: 1740-4. PMID: 8639653
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin V Antibody (H-3) | sc-74438 | 200 µg/ml | $310.00 | |||
Annexin V Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-525709 | 200 µg Ab; 10 µg BP | $348.00 | |||
Annexin V Antibody (H-3): m-IgG2a BP-HRP Bundle | sc-546371 | 200 µg Ab; 10 µg BP | $348.00 | |||
Annexin V Antibody (H-3) AC | sc-74438 AC | 500 µg/ml, 25% agarose | $408.00 | |||
Annexin V Antibody (H-3) HRP | sc-74438 HRP | 200 µg/ml | $310.00 | |||
Annexin V Antibody (H-3) FITC | sc-74438 FITC | 200 µg/ml | $324.00 | |||
Annexin V Antibody (H-3) PE | sc-74438 PE | 200 µg/ml | $337.00 | |||
Annexin V Antibody (H-3) Alexa Fluor® 488 | sc-74438 AF488 | 200 µg/ml | $350.00 | |||
Annexin V Antibody (H-3) Alexa Fluor® 546 | sc-74438 AF546 | 200 µg/ml | $350.00 | |||
Annexin V Antibody (H-3) Alexa Fluor® 594 | sc-74438 AF594 | 200 µg/ml | $350.00 | |||
Annexin V Antibody (H-3) Alexa Fluor® 647 | sc-74438 AF647 | 200 µg/ml | $350.00 | |||
Annexin V Antibody (H-3) Alexa Fluor® 680 | sc-74438 AF680 | 200 µg/ml | $350.00 | |||
Annexin V Antibody (H-3) Alexa Fluor® 790 | sc-74438 AF790 | 200 µg/ml | $350.00 |