β Enolase Antibody (XX-10): sc-100811

β Enolase Antibody (XX-10) is an IgG_2a κ mouse monoclonal beta Enolase antibody (also designated ENO3 antibody, Beta-enolase antibody, Enolase 3 antibody or Muscle-specific enolase antibody) that detects the beta Enolase protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. β Enolase Antibody (XX-10) is available as the non-conjugated anti-beta Enolase antibody. Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified: α Enolase, β Enolase and γ Enolase. α Enolase expression has been detected in most tissues, whereas β Enolase is expressed predominantly in muscle tissue and γ Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. β Enolase, also known as Enolase 3 or MSE (muscle-specific enolase), localizes to the cytoplasm and is expressed as a homodimer or a heterodimer with α Enolase in adult skeletal muscle. Mutations in the gene encoding β Enolase may result in glycogenesis type XIII (muscle-specific β enolase deficiency), a disorder characterized by fatigability, muscle weakness and exercise-induced myalgia (or muscle pain).

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References:

1. Beta-enolase deficiency, a new metabolic myopathy of distal glycolysis.  |  Comi, GP., et al. 2001. Ann Neurol. 50: 202-7. PMID: 11506403
2. Enolase: human tissue distribution and evidence for three different loci.  |  Chen, SH. and Giblett, ER. 1976. Ann Hum Genet. 39: 277-80. PMID: 1275442
3. Synapse formation is related to the onset of neuron-specific enolase immunoreactivity in the avian auditory and vestibular systems.  |  Whitehead, MC., et al. 1982. Dev Neurosci. 5: 298-307. PMID: 7140580
4. DNA sequences encoding enolase are remarkably conserved from yeast to mammals.  |  Verma, M. and Dutta, SK. 1994. Life Sci. 55: 893-9. PMID: 7520111
5. Coexpression of alpha and gamma enolase genes in neurons of adult rat brain.  |  Keller, A., et al. 1994. J Neurosci Res. 38: 493-504. PMID: 7815469
6. A comparative study of the distribution of alpha- and gamma-enolase subunits in cultured rat neural cells and fibroblasts.  |  Deloulme, JC., et al. 1997. Int J Dev Neurosci. 15: 183-94. PMID: 9178037
7. Expression of two neuronal markers, growth-associated protein 43 and neuron-specific enolase, in rat glial cells.  |  Sensenbrenner, M., et al. 1997. J Mol Med (Berl). 75: 653-63. PMID: 9351704
8. Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution.  |  Zhang, E., et al. 1997. Biochemistry. 36: 12526-34. PMID: 9376357

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β Enolase Antibody (XX-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-β Enolase Antibody (XX-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β Enolase Antibody (XX-10) Specifications

Alternate Product Name

ENO3

Product Clone Name

XX-10

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

228-278

Epitope Target

β Enolase

Epitope Species

human

Product Reactivity/Detection

β Enolase (muscle)

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

5 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

N/A

The preferred Enolase antibody is Enolase Antibody (A-5): sc-271384.