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CPTI Antibody (E-7): sc-393070
- CPTI Antibody (E-7) is a mouse monoclonal IgG1 κ CPTI antibody, cited in 32 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 665-688 within a C-terminal cytoplasmic domain of CPTI of human origin
- CPTI Antibody (E-7) is recommended for detection of CPTI, liver isoform (CPTI-L) and muscle isoform (CPTI-M) of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-CPTI Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CPTI (E-7): sc-393070.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CPTI Antibody (E-7) for WB applications. These reagents are now offered in bundles with CPTI Antibody (E-7) (see ordering information below).
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CPTI Antibody (E-7) is an IgG1 κ mouse monoclonal CPTI antibody (also designated CPT1A antibody, carnitine palmitoyltransferase 1A (liver) antibody, L-CPT1 antibody, CPT1-L antibody, carnitine/choline acetyltransferase antibody, CPT1B antibody, carnitine palmitoyltransferase 1B antibody, carnitine palmitoyltransferase 1B (muscle) antibody, CPT1M antibody, or CPT1-M antibody) that detects the CPTI protein of mouse, rat and human origin by WB, IP, IF and ELISA. CPTI Antibody (E-7) is available as both the non-conjugated anti-CPTI antibody form, as well as multiple conjugated forms of anti-CPTI antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The mitochondrial β-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Functional characterization of mammalian mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris. | Woldegiorgis, G., et al. 2000. J Nutr. 130: 310S-314S. PMID: 10721894
- Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system. | Woeltje, KF., et al. 1990. J Biol Chem. 265: 10714-9. PMID: 2355017
- Regulation of ketogenesis and the renaissance of carnitine palmitoyltransferase. | McGarry, JD., et al. 1989. Diabetes Metab Rev. 5: 271-84. PMID: 2656156
- Human liver mitochondrial carnitine palmitoyltransferase I: characterization of its cDNA and chromosomal localization and partial analysis of the gene. | Britton, CH., et al. 1995. Proc Natl Acad Sci U S A. 92: 1984-8. PMID: 7892212
- Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I. | Yamazaki, N., et al. 1996. Biochim Biophys Acta. 1307: 157-61. PMID: 8679700
- Structural features of the gene encoding human muscle type carnitine palmitoyltransferase I. | Yamazaki, N., et al. 1997. FEBS Lett. 409: 401-6. PMID: 9224698
- Functional studies of yeast-expressed human heart muscle carnitine palmitoyltransferase I. | Zhu, H., et al. 1997. Arch Biochem Biophys. 347: 53-61. PMID: 9344464
- Characterization of carnitine acylcarnitine translocase system of heart mitochondria. | Pande, SV. and Parvin, R. 1976. J Biol Chem. 251: 6683-91. PMID: 977593
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPTI Antibody (E-7) | sc-393070 | 200 µg/ml | $310.00 | |||
CPTI Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-530344 | 200 µg Ab; 10 µg BP | $348.00 | |||
CPTI Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-523791 | 200 µg Ab, 40 µg BP | $348.00 | |||
CPTI Antibody (E-7): m-IgG1 BP-HRP Bundle | sc-544000 | 200 µg Ab; 20 µg BP | $348.00 | |||
CPTI Antibody (E-7) AC | sc-393070 AC | 500 µg/ml, 25% agarose | $408.00 | |||
CPTI Antibody (E-7) HRP | sc-393070 HRP | 200 µg/ml | $310.00 | |||
CPTI Antibody (E-7) FITC | sc-393070 FITC | 200 µg/ml | $324.00 | |||
CPTI Antibody (E-7) PE | sc-393070 PE | 200 µg/ml | $337.00 | |||
CPTI Antibody (E-7) Alexa Fluor® 488 | sc-393070 AF488 | 200 µg/ml | $350.00 | |||
CPTI Antibody (E-7) Alexa Fluor® 546 | sc-393070 AF546 | 200 µg/ml | $350.00 | |||
CPTI Antibody (E-7) Alexa Fluor® 594 | sc-393070 AF594 | 200 µg/ml | $350.00 | |||
CPTI Antibody (E-7) Alexa Fluor® 647 | sc-393070 AF647 | 200 µg/ml | $350.00 | |||
CPTI Antibody (E-7) Alexa Fluor® 680 | sc-393070 AF680 | 200 µg/ml | $350.00 | |||
CPTI Antibody (E-7) Alexa Fluor® 790 | sc-393070 AF790 | 200 µg/ml | $350.00 | |||
CPTI (E-7) Neutralizing Peptide | sc-393070 P | 100 µg/0.5 ml | $67.00 |