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Cbl Antibody (A-9): sc-1651
- Cbl Antibody (A-9) is a mouse monoclonal IgG1 κ Cbl antibody, cited in 50 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 892-906 at the C-terminus of Cbl of human origin
- Cbl Antibody (A-9) is recommended for detection of Cbl of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-Cbl Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cbl (A-9): sc-1651.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Cbl Antibody (A-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Cbl Antibody (A-9) (see ordering information below).
Cbl Antibody (A-9) is an IgG1 κ mouse monoclonal Cbl antibody (also designated Cbl antibody or c-Cbl antibody) that detects Cbl of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Cbl Antibody (A-9) is available as the non-conjugated anti-Cbl antibody form, as well as multiple conjugated forms of anti-Cbl antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias; c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4;11) or t(11;14) abnormalities. Two proteins related to c-Cbl have been identified as Cbl-b (RING finger protein 56) and Cbl-3 (RING finger protein 57). Cbl-b has a proline-rich domain, a nuclear localization signal, a C3HC4 zinc finger and a putative leucine zipper. Cbl-b is expressed in normal and malignant mammary epithelial cells, various normal tissues and hematopoietic tissue and cell lines. Data suggests that Cbl-b encodes a protein that can interact with signal transduction proteins to regulate their function or be regulated by them.≠
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Relationship of the human protooncogene CBL2 on 11q23 to the t(4;11), t(11;22), and t(11;14) breakpoints. | Savage, PD., et al. 1991. Cytogenet Cell Genet. 56: 112-5. PMID: 2013228
- The sequences of the human and mouse c-cbl proto-oncogenes show v-cbl was generated by a large truncation encompassing a proline-rich domain and a leucine zipper-like motif. | Blake, TJ., et al. 1991. Oncogene. 6: 653-7. PMID: 2030914
- The c-cbl proto-oncogene is preferentially expressed in thymus and testis tissue and encodes a nuclear protein. | Langdon, WY., et al. 1989. J Virol. 63: 5420-4. PMID: 2585608
- Identification of two murine loci homologous to the v-cbl oncogene. | Regnier, DC., et al. 1989. J Virol. 63: 3678-82. PMID: 2760978
- v-cbl, an oncogene from a dual-recombinant murine retrovirus that induces early B-lineage lymphomas. | Langdon, WY., et al. 1989. Proc Natl Acad Sci U S A. 86: 1168-72. PMID: 2784003
- Prognostic significance of chromosomal abnormalities in acute leukaemias and myelodysplastic syndromes. | Yunis, JJ. and Brunning, RD. 1986. Clin Haematol. 15: 597-620. PMID: 3536236
- Formation of c-Cbl.phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. | Hartley, D. and Corvera, S. 1996. J Biol Chem. 271: 21939-43. PMID: 8702998
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cbl Antibody (A-9) | sc-1651 | 200 µg/ml | $310.00 | |||
Cbl Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-526471 | 200 µg Ab; 10 µg BP | $348.00 | |||
Cbl Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-531844 | 200 µg Ab; 20 µg BP | $348.00 | |||
Cbl Antibody (A-9) AC | sc-1651 AC | 500 µg/ml, 25% agarose | $408.00 | |||
Cbl Antibody (A-9) HRP | sc-1651 HRP | 200 µg/ml | $310.00 | |||
Cbl Antibody (A-9) FITC | sc-1651 FITC | 200 µg/ml | $324.00 | |||
Cbl Antibody (A-9) PE | sc-1651 PE | 200 µg/ml | $337.00 | |||
Cbl Antibody (A-9) Alexa Fluor® 488 | sc-1651 AF488 | 200 µg/ml | $350.00 | |||
Cbl Antibody (A-9) Alexa Fluor® 546 | sc-1651 AF546 | 200 µg/ml | $350.00 | |||
Cbl Antibody (A-9) Alexa Fluor® 594 | sc-1651 AF594 | 200 µg/ml | $350.00 | |||
Cbl Antibody (A-9) Alexa Fluor® 647 | sc-1651 AF647 | 200 µg/ml | $350.00 | |||
Cbl Antibody (A-9) Alexa Fluor® 680 | sc-1651 AF680 | 200 µg/ml | $350.00 | |||
Cbl Antibody (A-9) Alexa Fluor® 790 | sc-1651 AF790 | 200 µg/ml | $350.00 | |||
Cbl (A-9) Neutralizing Peptide | sc-1651 P | 100 µg/0.5 ml | $67.00 |