p-Cbl Antibody (E-10): sc-377571

p-Cbl Antibody (E-10) is an IgM κ mouse monoclonal p-Cbl antibody (also designated p-Cbl antibody or c-Cbl antibody) that detects Tyr 700 phosphorylated Cbl of mouse, rat and human origin by WB, IP, IF and ELISA. p-Cbl Antibody (E-10) is available as the non-conjugated anti-p-Cbl antibody form. Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias; c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4;11) or t(11;14) abnormalities. Two proteins related to c-Cbl have been identified as Cbl-b (RING finger protein 56) and Cbl-3 (RING finger protein 57). Cbl-b has a proline-rich domain, a nuclear localization signal, a C3HC4 zinc finger and a putative leucine zipper. Cbl-b is expressed in normal and malignant mammary epithelial cells, various normal tissues and hematopoietic tissue and cell lines. Data suggests that Cbl-b encodes a protein that can interact with signal transduction proteins to regulate their function or be regulated by them.

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References:

1. Relationship of the human protooncogene CBL2 on 11q23 to the t(4;11), t(11;22), and t(11;14) breakpoints.  |  Savage, PD., et al. 1991. Cytogenet Cell Genet. 56: 112-5. PMID: 2013228
2. The sequences of the human and mouse c-cbl proto-oncogenes show v-cbl was generated by a large truncation encompassing a proline-rich domain and a leucine zipper-like motif.  |  Blake, TJ., et al. 1991. Oncogene. 6: 653-7. PMID: 2030914
3. The c-cbl proto-oncogene is preferentially expressed in thymus and testis tissue and encodes a nuclear protein.  |  Langdon, WY., et al. 1989. J Virol. 63: 5420-4. PMID: 2585608
4. Identification of two murine loci homologous to the v-cbl oncogene.  |  Regnier, DC., et al. 1989. J Virol. 63: 3678-82. PMID: 2760978
5. v-cbl, an oncogene from a dual-recombinant murine retrovirus that induces early B-lineage lymphomas.  |  Langdon, WY., et al. 1989. Proc Natl Acad Sci U S A. 86: 1168-72. PMID: 2784003
6. Prognostic significance of chromosomal abnormalities in acute leukaemias and myelodysplastic syndromes.  |  Yunis, JJ. and Brunning, RD. 1986. Clin Haematol. 15: 597-620. PMID: 3536236
7. Formation of c-Cbl.phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism.  |  Hartley, D. and Corvera, S. 1996. J Biol Chem. 271: 21939-43. PMID: 8702998

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Cbl Antibody (E-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Cbl Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Cbl Antibody (E-10) Specifications

Alternate Product Name

c-Cbl

Product Clone Name

E-10

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgM kappa light chain

Amino Acids

Tyr 700

Epitope Target

Cbl

Epitope Species

human

Product Reactivity/Detection

Tyr 700 phosphorylated Cbl

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

porcine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

4 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

P: Blocking Peptide

The preferred Cbl antibody is Cbl Antibody (A-9): sc-1651.