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Crk I/II Antibody (D-6): sc-393160
- Crk I/II Antibody (D-6) is a mouse monoclonal IgG1 κ Crk I/II antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 109-132 within an internal region of Crk I of human origin
- recommended for detection of Crk I and Crk II of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Crk I/II Antibody (D-6) for WB applications. These reagents are now offered in bundles with Crk I/II Antibody (D-6) (see ordering information below).
Crk I/II Antibody (D-6) is an IgG1 κ mouse monoclonal Crk I/II antibody (also designated CRK Proto-Oncogene Adaptor Protein antibody, V-Crk Avian Sarcoma Virus antibody, Adapter Molecule Crk antibody, Proto-Oncogene C-Crk antibody, P38 antibody, V-Crk Sarcoma Virus CT10 Oncogene-Like Protein or CRKII antibody) that detects the Crk I/II protein of mouse, rat and human origin by WB, IP, IF and ELISA. Crk I/II Antibody (D-6) is available as the non-conjugated anti-Crk I/II antibody. The Crk family of adapter proteins including Crk-II, Crk-I, and Crk-L consist mostly of SH2 and SH3 domains. Through the interactions between SH2 domain and phosphotyrosine residues and/or between SH3 domain and proline-rich motifs, they are involved in a variety of signaling cascades. Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities (2-4). Crk-II has less transforming activity than Crk-I, although both Crk-I and Crk-II bind to many tyrosine-phosphorylated proteins that bind to grb2 (2,4). In addition, Crk-II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway. The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers.
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References:
- Mutant mice lacking Crk-II caused by the gene trap insertional mutagenesis: Crk-II is not essential for embryonic development. | Imaizumi, T., et al. 1999. Biochem Biophys Res Commun. 266: 569-74. PMID: 10600543
- Two species of human CRK cDNA encode proteins with distinct biological activities. | Matsuda, M., et al. 1992. Mol Cell Biol. 12: 3482-9. PMID: 1630456
- CRK proto-oncogene maps to human chromosome band 17p13. | Fioretos, T., et al. 1993. Oncogene. 8: 2853-5. PMID: 8378094
- Interaction in vitro of the product of the c-Crk-II proto-oncogene with the insulin-like growth factor I receptor. | Koval, AP., et al. 1998. Biochem J. 330 (Pt 2): 923-32. PMID: 9480911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Crk I/II Antibody (D-6) | sc-393160 | 200 µg/ml | $310.00 | |||
Crk I/II Antibody (D-6): m-IgG Fc BP-HRP Bundle | sc-538159 | 200 µg Ab; 10 µg BP | $348.00 | |||
Crk I/II Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-535719 | 200 µg Ab; 40 µg BP | $348.00 | |||
Crk I/II Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-545552 | 200 µg Ab; 20 µg BP | $348.00 | |||
Crk I/II (D-6) Neutralizing Peptide | sc-393160 P | 100 µg/0.5 ml | $67.00 |