CYP2A6 Antibody (F16 P2 D8): sc-53615

CYP2A6 Antibody (F16 P2 D8) is an IgG₁ κ mouse monoclonal CYP2A6 antibody (also designated CYP2A6 antibody) that detects the CYP2A6 protein of human origin by WB, IP, IF and IHC(P). CYP2A6 Antibody (F16 P2 D8) is available as both the non-conjugated anti-CYP2A6 antibody form, as well as multiple conjugated forms of anti-CYP2A6 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP2A6 is a liver enzyme that metabolizes a number of drugs and a variety of procarcinogens, though it is primarily responsible for the metabolism of nicotine, the major addictive agent in tobacco. CYP2A6 inactivates nicotine to cotinine, and then cotinine to 3-hydroxycotinine. Differences in CYP2A6 genotypes are related to nicotine dependence, and may influence smoking habits and withdrawal symptoms in individuals that are quitting smoking. This suggests that an individualized smoking cessation program may be designed based on CYP2A6 genotypes.

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References:

1. Novel human CYP2A6 alleles confound gene deletion analysis.  |  Nakajima, M., et al. 2004. FEBS Lett. 569: 75-81. PMID: 15225612
2. Nicotine metabolism: the impact of CYP2A6 on estimates of additive genetic influence.  |  Swan, GE., et al. 2005. Pharmacogenet Genomics. 15: 115-25. PMID: 15861035
3. Cytochrome p450 profile of colorectal cancer: identification of markers of prognosis.  |  Kumarakulasingham, M., et al. 2005. Clin Cancer Res. 11: 3758-65. PMID: 15897573
4. Cyp2a6 is a principal enzyme involved in hydroxylation of 1,7-dimethylxanthine, a main caffeine metabolite, in humans.  |  Kimura, M., et al. 2005. Drug Metab Dispos. 33: 1361-6. PMID: 15980104
5. Inactivation of CYP2A6 and CYP2A13 during nicotine metabolism.  |  von Weymarn, LB., et al. 2006. J Pharmacol Exp Ther. 316: 295-303. PMID: 16188955
6. CYP2A6 polymorphisms are associated with nicotine dependence and influence withdrawal symptoms in smoking cessation.  |  Kubota, T., et al. 2006. Pharmacogenomics J. 6: 115-9. PMID: 16402086
7. Smoking cessation program and CYP2A6 polymorphism.  |  Ozaki, S., et al. 2006. Front Biosci. 11: 2590-7. PMID: 16720336
8. CYP2A6 genotype, phenotype, and the use of nicotine metabolites as biomarkers during ad libitum smoking.  |  Malaiyandi, V., et al. 2006. Cancer Epidemiol Biomarkers Prev. 15: 1812-9. PMID: 17035386
9. CYP2A6 genotype and the metabolism and disposition kinetics of nicotine.  |  Benowitz, NL., et al. 2006. Clin Pharmacol Ther. 80: 457-67. PMID: 17112802

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CYP2A6 Antibody (F16 P2 D8) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-CYP2A6 Antibody (F16 P2 D8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CYP2A6 Antibody (F16 P2 D8) Specifications

Alternate Product Name

CYP2A6

Product Clone Name

F16 P2 D8

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

Epitope Target

CYP2A6

Epitope Species

human

Product Reactivity/Detection

CYP2A6, CYP2A7, CYP2A13 and CYP2B6

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P)

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

9 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin