Dvl-1/Dishevelled 1/DVL1 Antibody (3F12): sc-8025

Dvl-1 Antibody (3F12) is an IgG_2a κ mouse monoclonal Dvl-1 antibody (also designated DVL1 antibody, or dishevelled segment polarity protein 1 antibody) that detects the Dvl-1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Dvl-1 Antibody (3F12) is available as both the non-conjugated anti-Dvl-1 antibody form, as well as multiple conjugated forms of anti-Dvl-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Mammalian homologs of the Drosophila dishevelled (Dsh) gene have been identified, including Dvl-1, Dvl-2 and Dvl-3. The mammalian dishevelled proteins contain three homologous domains, two of which are unrelated to any other known protein. The third region is homologous to the discs-large homology domain of Drosophila discs-large-1, a tumor suppressor protein. Like their Drosophila counterpart, the dishevelled proteins are thought to be involved in embryogenesis. Overexpression of Dvl-1 has been shown to inhibit the phosphorylation of tau by GSK-3β. This finding may prove to be important in Alzheimer′s studies, which have shown that tau is hyperphosphorylated. In Drosophila, Dsh is a component of the frizzled signaling pathway. Both mammalian dishevelled and frizzled proteins are components of the Wnt signalling pathway.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled.  |  Sussman, DJ., et al. 1994. Dev Biol. 166: 73-86. PMID: 7958461
2. Dishevelled is a component of the frizzled signaling pathway in Drosophila.  |  Krasnow, RE., et al. 1995. Development. 121: 4095-102. PMID: 8575310
3. Human homologue sequences to the Drosophila dishevelled segment-polarity gene are deleted in the DiGeorge syndrome.  |  Pizzuti, A., et al. 1996. Am J Hum Genet. 58: 722-9. PMID: 8644734
4. cDNA characterization and chromosomal mapping of two human homologues of the Drosophila dishevelled polarity gene.  |  Pizzuti, A., et al. 1996. Hum Mol Genet. 5: 953-8. PMID: 8817329
5. Isolation and characterization of mouse dishevelled-3.  |  Tsang, M., et al. 1996. Dev Dyn. 207: 253-62. PMID: 8922524
6. A frizzled homolog functions in a vertebrate Wnt signaling pathway.  |  Yang-Snyder, J., et al. 1996. Curr Biol. 6: 1302-6. PMID: 8939578
7. Human dishevelled genes constitute a DHR-containing multigene family.  |  Semënov, MV. and Snyder, M. 1997. Genomics. 42: 302-10. PMID: 9192851
8. Overexpression of the mouse dishevelled-1 protein inhibits GSK-3beta-mediated phosphorylation of tau in transfected mammalian cells.  |  Wagner, U., et al. 1997. FEBS Lett. 411: 369-72. PMID: 9271238

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) has 79 citations in a variety of scientific publications.

Primary antibodies like Anti-Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) Specifications

Alternate Product Name

DVL1

Product Clone Name

3F12

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

651-695

Epitope Target

Dvl-1

Epitope Species

mouse

Product Reactivity/Detection

Dvl-1

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

79 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Dvl/Dishevelled antibody is Dvl/Dishevelled Antibody (B-4): sc-166303.