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NG2 Antibody (G-9): sc-166251

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Datasheets
  • NG2 Antibody (G-9) is a mouse monoclonal IgG2a κ, cited in 10 publications, provided at 200 µg/ml
  • raised against amino acids 2023-2322 mapping at the C-terminus of NG2 of human origin
  • Anti-NG2 Antibody (G-9) is recommended for detection of NG2 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-NG2 Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NG2 (G-9): sc-166251.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NG2 Antibody (G-9) for WB applications. These reagents are now offered in bundles with NG2 Antibody (G-9) (see ordering information below).

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NG2 Antibody (G-9) is an IgG2a κ mouse monoclonal NG2 antibody that detects NG2 of mouse, rat and human origin by WB, IP, IF and ELISA. NG2 Antibody (G-9) is available as the non-conjugated anti-NG2 antibody form, as well as multiple conjugated forms of anti-NG2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. NG2 (also known as melanoma-associated chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MSK16 and MEL-CSPG) stabilizes cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. NG2 may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. Threonine 2256 phosphorylation of rat NG2 (Threonine 2252 phosphorylation of human NG2) leads to redistribution of NG2 on the surface of astrocytomas, polarization of the cell and a significant increase in cell motility. NG2 acts as a co-receptor for spreading and focal contact formation in association with α4 β1 integrin in malignant melanoma cells. NG2 is present on blood vessels throughout the rat embryo. Microvessels within the rat CNS express NG2 on endothelial cells, and outside the CNS, NG2 is present on smooth muscle cells. NG2 is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

NG2 Antibody (G-9)

sc-166251
200 µg/ml
$310.00

NG2 Antibody (G-9): m-IgG Fc BP-HRP Bundle

sc-528929
200 µg Ab; 10 µg BP
$348.00

NG2 Antibody (G-9): m-IgGκ BP-HRP Bundle

sc-521544
200 µg Ab, 40 µg BP
$348.00

NG2 Antibody (G-9): m-IgG2a BP-HRP Bundle

sc-547208
200 µg Ab; 10 µg BP
$348.00

NG2 Antibody (G-9) AC

sc-166251 AC
500 µg/ml, 25% agarose
$408.00

NG2 Antibody (G-9) HRP

sc-166251 HRP
200 µg/ml
$310.00

NG2 Antibody (G-9) FITC

sc-166251 FITC
200 µg/ml
$324.00

NG2 Antibody (G-9) PE

sc-166251 PE
200 µg/ml
$337.00

NG2 Antibody (G-9) Alexa Fluor® 488

sc-166251 AF488
200 µg/ml
$350.00

NG2 Antibody (G-9) Alexa Fluor® 546

sc-166251 AF546
200 µg/ml
$350.00

NG2 Antibody (G-9) Alexa Fluor® 594

sc-166251 AF594
200 µg/ml
$350.00

NG2 Antibody (G-9) Alexa Fluor® 647

sc-166251 AF647
200 µg/ml
$350.00

NG2 Antibody (G-9) Alexa Fluor® 680

sc-166251 AF680
200 µg/ml
$350.00

NG2 Antibody (G-9) Alexa Fluor® 790

sc-166251 AF790
200 µg/ml
$350.00

Hi, I used this antibody (1:500 dilution) for WB by testing on SK-MEL-28 and Hela under a reducing condition. I used Nupage gel Tris-acetate 3-8% and transferd to PVDF at 70V for 3h. I could not get any band. Could you please suggest solutions? Thanks!

Asked by: Jane Wang
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

Hi. Would you please assist me with the following question regarding SC-166251 NG2 (G-9) antibody. Does this antibody work in immunofluorescence on paraffin embedded samples? Please advise.

Asked by: Jim Tertipis
Thank you for your question. We have not yet tested our mouse monoclonal, NG2 Antibody (G-9): sc-166251, on formalin-fixed paraffin-embedded tissue sections so we do not know the antibody is suitable for this application. We currently recommend the antibody for IF, or immunofluorescence on fresh or frozen tissues or cells fixed in acetone or methanol.
Answered by: Technical Support
Date published: 2017-03-08

Are there any issues with using sc-166251: NG2 (G-9) mouse monoclonal primary antibody on mouse samples?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-166251 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
  • y_2024, m_5, d_24, h_6CST
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Rated 4 out of 5 by from Good signalI tried the free sample to detect mouse CSPG4/NG2 in murine cell lines in WB (1:500) and IF (1:200). Good signal, right band size in WB.
Date published: 2024-02-05
Rated 3 out of 5 by from 抗体背景比较深抗体背景颜色较深,阳性较弱。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。
Date published: 2018-03-13
Rated 5 out of 5 by from Nice Western Blot data of NG2 expression in SKNice Western Blot data of NG2 expression in SK-MEL-24 whole cell lysate. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Nice Western Blot data of NG2 expression in SKNice Western Blot data of NG2 expression in SK-MEL-24 whole cell lysate and rat brain tissue extract . -SCBT QC
Date published: 2014-11-28
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NG2 Antibody (G-9) is rated 4.3 out of 5 by 4.
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