SDHA Antibody (F-2): sc-390381

SDHA Antibody (F-2) is an IgG_2a κ mouse monoclonal SDHA antibody (also designated Succinate Dehydrogenase Complex Flavoprotein Subunit A antibody, Flavoprotein Subunit Of Complex II antibody, SDHF antibody, Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit Mitochondrial antibody, Succinate Dehydrogenase Complex Subunit A Flavoprotein (Fp) antibody, or Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit antibody) that detects the SDHA protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. SDHA Antibody (F-2) is available as both the non-conjugated anti-SDHA antibody form, as well as multiple conjugated forms of anti-SDHA antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Histochemical investigations on the presence of acetylcholinesterase and succinic dehydrogenase in fetal human spinal cord and brain stem at different stages of development.  |  Wolf, P., et al. 1975. Eur Neurol. 13: 31-46. PMID: 1126345
2. Classification of muscle fiber types based on succinic dehydrogenase and myofibrillar ATPase reactions in normal and randomly reinnervated rat skeletal muscles.  |  Rymaszewska-Kossakowska, T. and Sliwowski, A. 1977. Folia Histochem Cytochem (Krakow). 15: 43-8. PMID: 140839
3. Time course of changes in human skeletal muscle succinate dehydrogenase and cytochrome oxidase activities and maximal oxygen uptake with physical activity and inactivity.  |  Henriksson, J. and Reitman, JS. 1977. Acta Physiol Scand. 99: 91-7. PMID: 190867
4. Proteins of the inner membrane of Escherichia coli: identification of succinate dehydrogenase by polyacrylamide gel electrophoresis with sdh amber mutants.  |  Spencer, ME. and Guest, JR. 1974. J Bacteriol. 117: 947-53. PMID: 4591960
5. Mutation of a nuclear succinate dehydrogenase gene results in mitochondrial respiratory chain deficiency.  |  Bourgeron, T., et al. 1995. Nat Genet. 11: 144-9. PMID: 7550341
6. Human complex II (succinate-ubiquinone oxidoreductase): cDNA cloning of the flavoprotein (Fp) subunit of liver mitochondria.  |  Hirawake, H., et al. 1994. J Biochem. 116: 221-7. PMID: 7798181
7. The effects of different patterns of muscle activity on capillary density, mechanical properties and structure of slow and fast rabbit muscles.  |  Brown, MD., et al. 1976. Pflugers Arch. 361: 241-50. PMID: 943767
8. Effect of low dose x-irradiation on the succinate dehydrogenase activity of guinea pig, rat and mouse tissues.  |  Shah, VC., et al. 1976. Strahlentherapie. 152: 83-91. PMID: 951722
9. Gene mutations in the succinate dehydrogenase subunit SDHB cause susceptibility to familial pheochromocytoma and to familial paraganglioma.  |  Astuti, D., et al. 2001. Am J Hum Genet. 69: 49-54. PMID: 11404820

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SDHA Antibody (F-2) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-SDHA Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SDHA Antibody (F-2) Specifications

Alternate Product Name

Succinate Dehydrogenase

Product Clone Name

F-2

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

637-664

Epitope Target

SDHA

Epitope Species

human

Product Reactivity/Detection

precursor and mature SDHA

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

porcine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

17 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Kappa Light Chain Binding Proteins

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin