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Wnt-5a Antibody (A-5): sc-365370
- Wnt-5a Antibody (A-5) is a mouse monoclonal IgG2b κ Wnt-5a antibody, cited in 42 publications, provided at 200 µg/ml
- raised against amino acids 23-80 mapping near the N-terminus of Wnt-5a of human origin
- Wnt-5a Antibody (A-5) is recommended for detection of Wnt-5a of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Wnt-5a Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Wnt-5a (A-5): sc-365370.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Wnt-5a Antibody (A-5). This work is in progress.
Wnt-5a Antibody (A-5) is an IgG2b κ mouse monoclonal Wnt-5a antibody (also designated WNT5A antibody) that detects the Wnt-5a protein of mouse, rat and human origin by WB, IP, IF and ELISA. Wnt-5a Antibody (A-5) is available as both the non-conjugated anti-Wnt-5a antibody form, as well as multiple conjugated forms of anti-Wnt-5a antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene "wingless" and to its vertebrate ortholog, Int1, a mouse protooncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins, which have features typical of secreted growth factors. Northern blot analysis detects expression of Wnt-5a in brain, lung, and heart. At least five distinct Wnt-5a transcripts are observed, which are due to transcript variability 5-prime to the initiation methionine. In situ hybridization detects a complex spatial and temporal pattern of Wnt-5a in the mouse, including expression in the developing central nervous system, limbs, facial processes, and the posterior region of the fetus. Human frizzled-5 is the receptor for the Wnt-5a ligand. It is suggested that Wnt-5a augments primitive hematopoietic development in vivo and represents an in vivo regulator of hematopoietic stem cell function in the human.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo. | Murdoch, B., et al. 2003. Proc Natl Acad Sci U S A. 100: 3422-7. PMID: 12626754
- Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development. | Gavin, BJ., et al. 1990. Genes Dev. 4: 2319-32. PMID: 2279700
- Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21. | Clark, CC., et al. 1993. Genomics. 18: 249-60. PMID: 8288227
- A member of the Frizzled protein family mediating axis induction by Wnt-5A. | He, X., et al. 1997. Science. 275: 1652-4. PMID: 9054360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wnt-5a Antibody (A-5) | sc-365370 | 200 µg/ml | $310.00 | |||
Wnt-5a Antibody (A-5) AC | sc-365370 AC | 500 µg/ml, 25% agarose | $408.00 | |||
Wnt-5a Antibody (A-5) HRP | sc-365370 HRP | 200 µg/ml | $310.00 | |||
Wnt-5a Antibody (A-5) FITC | sc-365370 FITC | 200 µg/ml | $324.00 | |||
Wnt-5a Antibody (A-5) PE | sc-365370 PE | 200 µg/ml | $337.00 | |||
Wnt-5a Antibody (A-5) Alexa Fluor® 488 | sc-365370 AF488 | 200 µg/ml | $350.00 | |||
Wnt-5a Antibody (A-5) Alexa Fluor® 546 | sc-365370 AF546 | 200 µg/ml | $350.00 | |||
Wnt-5a Antibody (A-5) Alexa Fluor® 594 | sc-365370 AF594 | 200 µg/ml | $350.00 | |||
Wnt-5a Antibody (A-5) Alexa Fluor® 647 | sc-365370 AF647 | 200 µg/ml | $350.00 | |||
Wnt-5a Antibody (A-5) Alexa Fluor® 680 | sc-365370 AF680 | 200 µg/ml | $350.00 | |||
Wnt-5a Antibody (A-5) Alexa Fluor® 790 | sc-365370 AF790 | 200 µg/ml | $350.00 |