Wnt-7a Antibody (E-9): sc-365665

Wnt-7a Antibody (E-9) is an IgG₁ κ mouse monoclonal Wnt-7a antibody (also designated WNT7A antibody) that detects the Wnt-7a protein of mouse, rat and human origin by WB, IP, IF and ELISA. Wnt-7a Antibody (E-9) is available as both the non-conjugated anti-Wnt-7a antibody form, as well as multiple conjugated forms of anti-Wnt-7a antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. The Wnt family has two functional classes according to their biological activities; Wnts that signal through a Wnt-1/wingless pathway by stabilizing cytoplasmic beta-catenin, and Wnts that stimulate intracellular Ca2+ release and activate two kinases, CamKII and PKC, in a G protein-dependent manner. Wnt-7a guides the development of the anterior-posterior axis in the female reproductive tract, and influences uterine smooth muscle pattering and maintenance of adult uterine function. The human Wnt-7a gene maps to chromosome 3p25. The human Wnt-7b gene maps to chromosome 22q13.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The Wnt/Ca2+ pathway: a new vertebrate Wnt signaling pathway takes shape.  |  Kυhl, M., et al. 2000. Trends Genet. 16: 279-83. PMID: 10858654
2. Decreased expression of Wnt7a mRNA is inversely associated with the expression of estrogen receptor-alpha in human uterine leiomyoma.  |  Li, S., et al. 2001. J Clin Endocrinol Metab. 86: 454-7. PMID: 11232041
3. Isolation, characterization and chromosomal assignment of the human WNT7A gene.  |  Ikegawa, S., et al. 1996. Cytogenet Cell Genet. 74: 149-52. PMID: 8893824
4. Molecular models for vertebrate limb development.  |  Johnson, RL. and Tabin, CJ. 1997. Cell. 90: 979-90. PMID: 9323126

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Wnt-7a Antibody (E-9) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Wnt-7a Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Wnt-7a Antibody (E-9) Specifications

Alternate Product Name

WNT7A

Product Clone Name

E-9

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

247-273

Epitope Target

Wnt-7a

Epitope Species

human

Product Reactivity/Detection

precursor and mature Wnt-7a

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

equine, canine, porcine and avian

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

8 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Wnt-7a/b antibody is Wnt-7a/b Antibody (H-8): sc-365459.